线粒体蛋白质的生物合成：血红素对δ-氨基乙酰丙酸合酶成熟的调节

Biogenesis of mitochondrial proteins regulation of maturation of delta-aminolevulinate synthase by hemin.

作者信息

Ades I Z

出版信息

Biochem Biophys Res Commun. 1983 Jan 14;110(1):42-7. doi: 10.1016/0006-291x(83)91257-3.

Abstract

The effect of hemin on the biogenesis of delta-aminolevulinate (ALA) synthase was examined in primary cultures of chick embryo hepatocytes. Hemin (0.010 mM) in the culture medium significantly inhibited the induction of ALA synthase activity in hepatocytes exposed to the porphyrogenic agent allylisopropylacetamide. In hepatocytes pulse-labeled with [35S]methionine for 45 min in the presence and absence of hemin, it was determined by immunechemical analyses that hemin blocked the processing of the precursor of ALA synthase.

摘要

在鸡胚肝细胞原代培养物中研究了血红素对δ-氨基乙酰丙酸（ALA）合酶生物合成的影响。培养基中的血红素（0.010 mM）显著抑制了暴露于致卟啉剂烯丙基异丙基乙酰胺的肝细胞中ALA合酶活性的诱导。在用[35S]甲硫氨酸脉冲标记45分钟的肝细胞中，无论有无血红素，通过免疫化学分析确定血红素阻断了ALA合酶前体的加工过程。