Ramasharma K, Cabrera C M, Li C H
Biochem Biophys Res Commun. 1986 Oct 30;140(2):536-42. doi: 10.1016/0006-291x(86)90765-5.
Antisera raised in rabbits against synthetic insulin-like growth factor-II (IGF-II) were used to develop a specific radioimmunoassay (RIA) for IGF-II. Affinity purified antibodies showed 6% cross-reactivity with IGF-I but failed to recognize insulin even at 10 micrograms/tube. Utilizing this RIA system, immunoreactive IGF-II was identified in the pooled samples of human follicular fluid and seminal plasma. The acid-ethanol precipitates of human seminal and follicular fluids were chromatographed on Sephadex G-50 column and the IGF-II immunoreactive fractions were subjected to reversed-phase high performance liquid chromatography. It was found that immunoactive IGF-II was eluted in the same location as that of synthetic IGF-II. The data indicate for the first time that human seminal plasma and follicular fluid contain significant amounts of IGF-II.
用兔制备的抗合成胰岛素样生长因子-II（IGF-II）抗血清建立了一种针对IGF-II的特异性放射免疫分析（RIA）方法。亲和纯化的抗体与IGF-I有6%的交叉反应，但即使在每管10微克时也未能识别胰岛素。利用该RIA系统，在人卵泡液和精浆的混合样本中鉴定出免疫反应性IGF-II。人精液和卵泡液的酸乙醇沉淀物在Sephadex G-50柱上进行色谱分析，IGF-II免疫反应性组分进行反相高效液相色谱分析。发现免疫活性IGF-II与合成IGF-II在相同位置洗脱。这些数据首次表明人精浆和卵泡液中含有大量的IGF-II。
