DNA聚合酶β在体外的聚（ADP - 核糖基）化作用
Poly(ADP-ribosyl)ation of DNA polymerase beta in vitro.
作者信息
Ohashi Y, Itaya A, Tanaka Y, Yoshihara K, Kamiya T, Matsukage A
出版信息
Biochem Biophys Res Commun. 1986 Oct 30;140(2):666-73. doi: 10.1016/0006-291x(86)90783-7.
PMID:3778475
Abstract
DNA polymerase beta purified from bovine thymus is markedly inhibited when incubated in a reconstituted poly(ADP-ribosyl)ating reaction system. Analyses of the reaction product synthesized in this system by SDS-polyacrylamide gel electrophoresis and subsequent fluorography of the gel indicated that ADP-ribose is covalently attached to DNA polymerase beta molecule (Mr = 44,000).
摘要
从牛胸腺中纯化得到的DNA聚合酶β在重构的聚（ADP-核糖基）化反应体系中孵育时会受到显著抑制。通过SDS-聚丙烯酰胺凝胶电泳对该体系中合成的反应产物进行分析，并随后对凝胶进行荧光显影，结果表明ADP-核糖共价连接到了DNA聚合酶β分子（分子量 = 44,000）上。
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