Nomura H, Ase K, Sekiguchi K, Kikkawa U, Nishizuka Y, Nakano Y, Satoh T
Biochem Biophys Res Commun. 1986 Nov 14;140(3):1143-51. doi: 10.1016/0006-291x(86)90754-0.
In intact platelets, a permeable diacylglycerol having a 1,2-sn- but not 2,3-sn- configuration activated protein kinase C directly. In the presence of Ca2+-ionophore this diacylglycerol caused full activation of platelet release reaction. 1,3-Isomer was inactive. Among these isomers only 1,2-sn-diacylglycerol was converted rapidly to the corresponding phosphatidic acid in both intact and broken cell preparations. Thus, the diacylglycerol which functions in stimulus-response coupling possesses a 1,2-sn-glycerol backbone, and other isomers are not involved in the signal transduction through the protein kinase C pathway.
在完整的血小板中，具有1,2- sn -构型而非2,3- sn -构型的可渗透二酰基甘油可直接激活蛋白激酶C。在钙离子载体存在的情况下，这种二酰基甘油可使血小板释放反应完全激活。1,3-异构体无活性。在这些异构体中，只有1,2- sn -二酰基甘油在完整细胞和破碎细胞制剂中都能迅速转化为相应的磷脂酸。因此，在刺激-反应偶联中起作用的二酰基甘油具有1,2- sn -甘油主链，其他异构体不参与通过蛋白激酶C途径的信号转导。
