Zhao Nan, Niu Rui-Ze, Zhu Yu-Hang, Yu Chang-Yin
Animal Zoology Department Kunming Medical University Kunming Yunnan China.
Department of Anesthesiology Affiliated Stomatology Hospital of Zunyi Medical University Zunyi Guizhou China.
Ibrain. 2021 Mar 28;7(1):12-20. doi: 10.1002/j.2769-2795.2021.tb00059.x. eCollection 2021 Mar.
Cerebral stroke is the second leading cause of death with high mortality and morbidity worldwide, currently it lacks effective therapies to improve the prognosis. This study was aimed to explore the role of bone marrow mesenchymal stem cells (BMSCs) transplantation in the recovery of brain structure and function after ischemic cerebral infarction by magnetic resonance imaging (MRI).
By applying internal carotid artery embolization, the ischemic cerebral infarction model in rats was established. MRI was performed to detect the imaging changes in the brain tissue after modeling, and the successful modeling was evidenced by the presence of obvious high-signal infarct areas in the brain. BMSCs were then injected into the lateral ventricles of rats, and the recovery of brain tissue and function were quantitatively evaluated by T2-weighted image (T2WI) and voxel-based morphology (VBM) after 28 days.
The results showed that BMSCs were cell subsets with multiple differentiation potentials. Deficits caused by Ischemic cerebral infarction were relieved by BMSCs transplantation, including increase in damaged cerebral tissue and recovery of cerebral function. In addition, the combined imaging technology of VBM and T2WI quantitatively revealed the effectiveness of BMSCs in repairing damaged brain tissue structure and function.
Taken together, the results revealed that the transplantation of BMSCs into the lateral ventricle was beneficial to repair the structure and function of the damaged brain tissue after ischemic cerebral infarction. Moreover, the combination of VBM and T2WI technology can detect the level of brain injury in ischemic cerebral infarction dynamically and noninvasively, and evaluate the recovery of structure and function of damaged brain tissue.
脑卒中是全球第二大致死原因，死亡率和发病率都很高，目前缺乏有效的治疗方法来改善预后。本研究旨在通过磁共振成像（MRI）探讨骨髓间充质干细胞（BMSCs）移植在缺血性脑梗死后脑结构和功能恢复中的作用。
通过颈内动脉栓塞法建立大鼠缺血性脑梗死模型。建模后进行MRI检测脑组织的影像学变化，脑内出现明显的高信号梗死灶证明建模成功。然后将BMSCs注入大鼠侧脑室，28天后通过T2加权成像（T2WI）和基于体素的形态学分析（VBM）对脑组织和功能的恢复进行定量评估。
结果表明，BMSCs是具有多种分化潜能的细胞亚群。BMSCs移植可减轻缺血性脑梗死所致的缺损，包括受损脑组织增加和脑功能恢复。此外，VBM和T2WI联合成像技术定量显示了BMSCs在修复受损脑组织结构和功能方面的有效性。
综上所述，结果表明将BMSCs移植到侧脑室有利于修复缺血性脑梗死受损脑组织的结构和功能。此外，VBM和T2WI技术联合应用可动态、无创地检测缺血性脑梗死的脑损伤程度，并评估受损脑组织结构和功能的恢复情况。
