Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 201900, China.
Shanghai 6th People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Contrast Media Mol Imaging. 2019 Aug 29;2019:5184105. doi: 10.1155/2019/5184105. eCollection 2019.
We aimed to identify a suitable method for long-term monitoring of the migration and proliferation of mesenchymal stromal cells in stroke models of rats using ferritin transgene expression by magnetic resonance imaging (MRI). Bone marrow mesenchymal stromal cells (BMSCs) were transduced with a lentivirus containing a shuttle plasmid (pCDH-CMV-MCS-EF1-copGFP) carrying the ferritin heavy chain 1 () gene. Ferritin expression in stromal cells was evaluated with western blotting and immunofluorescent staining. The iron uptake of -BMSCs was measured with Prussian blue staining. Following surgical introduction of middle cerebral artery occlusion, -BMSCs and superparamagnetic iron oxide- (SPIO-) labeled BMSCs were injected through the internal jugular vein. The imaging and signal intensities were monitored by diffusion-weighted imaging (DWI), T2-weighted imaging (T2WI), and susceptibility-weighted imaging (SWI) and . Pathology was performed for comparison. We observed that the MRI signal intensity of SPIO-BMSCs gradually reduced over time. -BMSCs showed the same signal intensity between 10 and 60 days. SWI showed hypointense lesions in the SPIO-BMSC (traceable for 30 d) and -BMSC groups. T2WI was not sensitive enough to trace -BMSCs. After transplantation, Prussian blue-stained cells were observed around the infarction area and in the infarction center in both transplantation models. -BMSCs transplanted for treating focal cerebral infarction were safe, reliable, and traceable by MRI. labeling was more stable and suitable than SPIO labeling for long-term tracking. SWI was more sensitive than T2W1 and suitable as the optimal MRI-tracking sequence.
我们旨在通过磁共振成像（MRI）检测铁蛋白转基因表达来鉴定一种适用于大鼠中风模型中间质基质细胞迁移和增殖的长期监测方法。骨髓间充质基质细胞（BMSCs）用携带铁蛋白重链 1（）基因的慢病毒转导。通过 Western blot 和免疫荧光染色评估基质细胞中的铁蛋白表达。用普鲁士蓝染色测量-BMSCs 的铁摄取。在大脑中动脉闭塞手术后，通过颈内静脉注射 -BMSCs 和超顺磁性氧化铁（SPIO）标记的 BMSCs。通过弥散加权成像（DWI）、T2 加权成像（T2WI）和磁敏感加权成像（SWI）监测成像和信号强度，并且进行病理学比较。我们观察到 SPIO-BMSCs 的 MRI 信号强度随时间逐渐降低。-BMSCs 在 10 至 60 天之间显示相同的信号强度。SWI 显示 SPIO-BMSC（可追踪 30d）和 -BMSC 组中的低信号病变。T2WI 不够敏感，无法追踪 -BMSCs。移植后，在两种移植模型的梗塞区域周围和梗塞中心观察到普鲁士蓝染色的细胞。用于治疗局灶性脑梗死的 -BMSCs 是安全、可靠的，并且可以通过 MRI 进行追踪。与 SPIO 标记相比，标记更稳定，更适合长期追踪。SWI 比 T2W1 更敏感，是最佳的 MRI 追踪序列。
