Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen N, Denmark; Department of Ophthalmology, Copenhagen University Hospital, Rigshospitalet, Valdemar Hansens Vej 1-23, 2600 Glostrup, Denmark.
Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen N, Denmark.
J Optom. 2024 Jan-Mar;17(1):100481. doi: 10.1016/j.optom.2023.100481. Epub 2023 Oct 1.
To investigate the effect of benzalkonium chloride (BAK)-preserved latanoprost and bimatoprost, polyquad (PQ)-preserved travoprost, and preservative-free (PF) latanoprost and tafluprost, all prostaglandin analogues (PGAs), on human conjunctival goblet cell (GC) survival. Furthermore, to investigate the effect of BAK-preserved and PF latanoprost on the cytokine secretion from GC.
Primary human conjunctival GCs were cultivated from donor tissue. Lactate dehydrogenase (LDH) and tetrazolium dye colorimetric (MTT) assays were used for the assessment of GC survival. A cytometric bead array was employed for measuring secretion of interleukin (IL)-6 and IL-8 from GC.
BAK-preserved latanoprost and bimatoprost reduced cell survival by 28% (p = 0.0133) and 20% (p = 0.0208), respectively, in the LDH assay compared to a negative control. BAK-preserved latanoprost reduced cell proliferation by 54% (p = 0.003), BAK-preserved bimatoprost by 45% (p = 0.006), PQ-preserved travoprost by 16% (p = 0.0041), and PF latanoprost by 19% (p = 0.0001), in the MTT assay compared to a negative control. Only PF tafluprost did not affect the GCs in either assay. BAK-preserved latanoprost caused an increase in the secretion of pro-inflammatory IL-6 and IL-8 (p = 0.0001 and p = 0.0019, respectively) compared to a negative control, which PF latanoprost did not.
BAK-preserved PGA eye drops were more cytotoxic to GCs than PQ-preserved and PF PGA eye drops. BAK-preserved latanoprost induced an inflammatory response in GC. Treatment with PF and PQ-preserved PGA eye drops could mean better tolerability and adherence in glaucoma patients compared to treatment with BAK-preserved PGA eye drops.
研究苯扎氯铵（BAK）保存的拉坦前列素和贝美前列素、多聚季铵（PQ）保存的曲伏前列素以及无防腐剂（PF）保存的拉坦前列素和他氟前列素，所有这些前列腺素类似物（PGAs）对人结膜杯状细胞（GC）存活的影响。此外，还研究了 BAK 保存的和 PF 拉坦前列素对 GC 细胞因子分泌的影响。
从供体组织中培养原代人结膜 GC。使用乳酸脱氢酶（LDH）和四唑染料比色（MTT）测定法评估 GC 的存活率。采用细胞因子微珠阵列法测量 GC 分泌白细胞介素（IL）-6 和 IL-8 的情况。
与阴性对照组相比，BAK 保存的拉坦前列素和贝美前列素在 LDH 测定中使细胞存活率分别降低了 28%（p=0.0133）和 20%（p=0.0208）。BAK 保存的拉坦前列素使细胞增殖减少了 54%（p=0.003），BAK 保存的贝美前列素减少了 45%（p=0.006），PQ 保存的曲伏前列素减少了 16%（p=0.0041），PF 拉坦前列素减少了 19%（p=0.0001），在 MTT 测定中与阴性对照组相比。只有 PF 他氟前列素在这两种测定中均未影响 GC。与阴性对照组相比，BAK 保存的拉坦前列素导致促炎细胞因子 IL-6 和 IL-8 的分泌增加（p=0.0001 和 p=0.0019），而 PF 拉坦前列素则没有。
与 PQ 保存的和 PF 的 PGA 滴眼液相比，BAK 保存的 PGA 滴眼液对 GCs 的细胞毒性更大。BAK 保存的拉坦前列素诱导 GC 产生炎症反应。与 BAK 保存的 PGA 滴眼液相比，使用 PF 和 PQ 保存的 PGA 滴眼液治疗可能意味着青光眼患者的耐受性和依从性更好。
