Jan K M
Biorheology. 1986;23(2):91-8. doi: 10.3233/bir-1986-23202.
Red blood cell (RBC) aggregation in heparin-saline solution was quantified by microscopic observation. The adsorption isotherms of heparin onto normal and neuraminidase-treated RBC surfaces were determined by radioactive heparin labeled with 125I-Bolton-Hunter Reagent. RBC aggregation by heparin requires the presence of sialic acids at cell surface and was enhanced by reduction of ionic strength of the suspending medium. Adsorption of heparin onto RBC surface was increased by removal of sialic acids. These findings not only serve to elucidate the basic mechanism of cell-cell interaction mediated by negatively charged macromolecules, but also provide experimental evidence for the possible conformational change of macromolecules at the charged surface.
通过显微镜观察对肝素盐溶液中的红细胞（RBC）聚集进行了定量分析。用125I-博尔顿-亨特试剂标记的放射性肝素测定肝素在正常和经神经氨酸酶处理的RBC表面的吸附等温线。肝素引起的RBC聚集需要细胞表面存在唾液酸，并且通过降低悬浮介质的离子强度而增强。去除唾液酸可增加肝素在RBC表面的吸附。这些发现不仅有助于阐明由带负电荷的大分子介导的细胞间相互作用的基本机制，而且为大分子在带电表面可能的构象变化提供了实验证据。
