Yunnan Academy of Agricultural Sciences, Food Crops Research Institute, Kunming, China.
Yunnan Grain Industry Group Co., Ltd, Kunming, China.
PeerJ. 2023 Sep 29;11:e16168. doi: 10.7717/peerj.16168. eCollection 2023.
The domain of unknown function 560 (DUF560), also known as the PDDEXK_6 family, is a ubiquitous plant protein that has been confirmed to play critical roles in Arabidopsis root development as well as ABA and abiotic responses. However, genome-wide identification and expression pattern analysis in rice () still need to be improved. Based on the phylogenetic relationship, 10 genes were identified and classified into four subfamilies. Segmental duplication was essential to the expansion of , which were subjected to purifying selective pressure. Except for and , the shared colinear gene pairs with five monocot species, showing that they were conserved in evolution. Furthermore, the conserved domains, gene structures, SNPs distribution, and targeting miRNAs were systematically investigated. Massive cis-regulatory elements were discovered in promoter regions, implying that may be important in hormone regulation and abiotic stress response. Therefore, we analyzed plant hormone-induced transcriptome data and performed qRT-PCR on eight under drought, cold, and phosphorus-deficient stresses. The results revealed that most respond to ABA and JA treatment, as well as drought and cold conditions. In conclusion, our findings provided insights into the evolution and function of , which could benefit crop breeding in the future.
未知功能域 560（DUF560），也称为 PDDEXK_6 家族，是一种普遍存在的植物蛋白，已被证实在拟南芥根发育以及 ABA 和非生物响应中发挥关键作用。然而，在水稻（）中，仍需要改进全基因组鉴定和表达模式分析。基于系统发生关系，鉴定到 10 个基因，并将其分为四个亚家族。片段复制对于的扩张至关重要，这受到纯化选择压力的影响。除了和，与五个单子叶植物物种共享共线性基因对，表明它们在进化中是保守的。此外，还系统地研究了保守结构域、基因结构、SNP 分布和靶向 miRNAs。在启动子区域发现了大量的顺式调控元件，表明可能在激素调节和非生物胁迫响应中起重要作用。因此，我们分析了植物激素诱导的转录组数据，并对 8 个在干旱、寒冷和缺磷胁迫下进行 qRT-PCR 检测。结果表明，大多数对 ABA 和 JA 处理以及干旱和寒冷条件有反应。总之，我们的研究结果为提供了进化和功能方面的见解，这将有助于未来的作物育种。
