InBioS-Center for Protein Engineering, Institut de Chimie B6a, University of Liège , Liège, Belgium.
Department of Biology, California State University , Bakersfield, California, USA.
Microbiol Spectr. 2023 Dec 12;11(6):e0197523. doi: 10.1128/spectrum.01975-23. Epub 2023 Oct 4.
Common scab is a disease caused by a few species that affects important root and tuber crops including potato, beet, radish, and parsnip, resulting in major economic losses worldwide. In this work, we unveiled the molecular basis of host recognition by these pathogens by solving the structure of the sugar-binding protein CebE of in complex with cellotriose, the main elicitor of the pathogenic lifestyle of these bacteria. We further revealed that the signaling pathway from CebE-mediated transport of cellotriose is conserved in all pathogenic species except which causes soft rot disease in sweet potatoes. Our work also provides the structural basis of the uptake of cellobiose and cellotriose in saprophytic species, the first step activating the expression of the enzymatic system degrading the most abundant polysaccharide on earth, cellulose.
普通结痂病是由少数几种真菌引起的一种疾病，影响包括马铃薯、甜菜、萝卜和欧防风在内的重要根茎作物，导致全球重大经济损失。在这项工作中，我们通过解析与纤维三糖这一病原菌致病生活方式的主要激发子复合物的结构，揭示了这些病原菌识别宿主的分子基础。我们还发现，纤维三糖经 CebE 介导的转运信号通路在除了引起甘薯软腐病的病原菌之外的所有病原菌种中都是保守的。我们的工作还为在腐生种中摄取纤维二糖和纤维三糖提供了结构基础，这是激活地球上最丰富的多糖——纤维素的酶系统表达的第一步。
