Institute of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, Tallinn, 12618, Estonia.
Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11-12, Braunschweig, 38102, Germany.
Arch Virol. 2023 Oct 4;168(10):265. doi: 10.1007/s00705-023-05893-5.
We report sequencing of four historical cynosurus mottle virus (CnMoV) isolates, originating from different hosts and locations. The CnMoV genome, ranging from 4417 to 4419 nt, encodes five ORFs. It shares 48.1% nucleotide sequence identity with cocksfoot mottle virus and 69.8% with the recently discovered Poaceae Liege sobemovirus. Phylogenetic analysis supports classification within the genus Sobemovirus. Sequenced CnMoV isolates exhibit 96.4-99.9% identity. Nucleotide substitutions leading to amino acid changes showed no host associations. However, amino acid changes in the coat protein appear to be linked to differences in serological properties. Aphid transmission tests confirmed non-transmissibility, consistent with earlier observations for the English isolate.
我们报告了四个历史上的雀稗斑驳病毒（CnMoV）分离株的测序结果，这些分离株起源于不同的宿主和地点。CnMoV 基因组大小为 4417 到 4419 个核苷酸，编码五个 ORF。它与车轴草斑驳病毒的核苷酸序列同一性为 48.1%，与最近发现的 Poaceae Liege sobemovirus 的核苷酸序列同一性为 69.8%。系统发育分析支持将其分类在 Sobemovirus 属内。测序的 CnMoV 分离株显示出 96.4-99.9%的同一性。导致氨基酸变化的核苷酸取代与宿主无关。然而，外壳蛋白中的氨基酸变化似乎与血清学特性的差异有关。蚜虫传播试验证实了非传染性，这与英国分离株的早期观察结果一致。
