Department of Ophthalmology, Erciyes University Medicine Faculty, Kayseri, Turkey.
Department of Histology and Embryology, Erciyes University Medicine Faculty, Kayseri, Turkey.
Tissue Cell. 2023 Dec;85:102226. doi: 10.1016/j.tice.2023.102226. Epub 2023 Sep 27.
This study aimed to investigate the protective effect of melatonin against the acute toxicity of cisplatin in ocular tissues. The eyes of 40 rats were divided into 4 groups: Control group (10 rats), Melatonin (Mel) group (10 rats), Cisplatin (Cis) group (10 rats), Melatonin (Mel) + Cisplatin (Cis) group (10 rats). Retina, cornea, and ciliary body tissues were examined after hematoxylin-eosin staining of sections obtained from the eyes and were scored for disorganization and degeneration. Apoptotic cells were counted for the retina, cornea, and ciliary body with the TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) method. The total antioxidant status (TAS) / total oxidant status (TOS) of homogenized eye tissues were measured. While apoptotic cells were found to increase in the cornea of the Cisplatin (Cis) group, no difference was found regarding the retina and ciliary body cell count. An increased number of apoptotic cells in the cornea of the Cis group was found while there was a decrease in the group where Cisplatin and Melatonin were administered together (Mel+Cis group). There was no statistically significant difference amongst groups for TOS or TAS. Melatonin had a partial protective effect against histological damage.
本研究旨在探讨褪黑素对眼部组织顺铂急性毒性的保护作用。将 40 只大鼠的眼睛分为 4 组：对照组（10 只大鼠）、褪黑素（Mel）组（10 只大鼠）、顺铂（Cis）组（10 只大鼠）、褪黑素（Mel）+顺铂（Cis）组（10 只大鼠）。对从眼睛中获得的切片进行苏木精-伊红染色后，检查视网膜、角膜和睫状体组织，并对组织紊乱和变性进行评分。用 TUNEL（末端脱氧核苷酸转移酶 dUTP 缺口末端标记）法对视网膜、角膜和睫状体中的凋亡细胞进行计数。测量匀浆眼组织的总抗氧化状态（TAS）/总氧化状态（TOS）。虽然在顺铂（Cis）组的角膜中发现凋亡细胞增加，但在视网膜和睫状体细胞计数方面没有差异。在同时给予顺铂和褪黑素的 Mel+Cis 组中，发现角膜中凋亡细胞数量增加，而在 Cis 组中则减少。TOS 或 TAS 各组之间无统计学差异。褪黑素对组织损伤有部分保护作用。
