Altern Ther Health Med. 2024 Jan;30(1):270-277.
Bronchial asthma is a prevalent respiratory disorder characterized by airway inflammation. This study aimed to investigate the protective effect of Pingchuanning decoction (PCN) on airway inflammation in bronchial asthma, focusing on the role of autophagy and its underlying molecular mechanism.
Using an in vitro lipopolysaccharide (LPS)-induced inflammatory damage model of human airway epithelial cells (16HBE), we assessed the effect of PCN. Various experiments were performed to evaluate the expression of autophagy-related genes, autophagosome and vesicle counts, and reactive oxygen species (ROS) levels.
First, PCN reduced LPS-induced cellular inflammation. Second, PCN decreased the number of autophagosomes and autophagic vesicles. And third, PCN significantly reduced reactive oxygen species (ROS) levels. Most importantly, PCN also down-regulated LPS-induced expression of HMGB1, Beclin-1, and autophagy-related gene 5 (ATG5) while enhancing the expression of B-cell lymphoma 2 (Bcl-2), which further reduced the LC3II/I ratio.
PCN reduces the 16HBE inflammatory response by inhibiting the overexpression of ROS/HMGB1/Beclin-1 mediated cell autophagy. Therefore, it may serve as a potential drug for treating bronchial asthma.
支气管哮喘是一种常见的呼吸道疾病，其特征为气道炎症。本研究旨在探讨平喘宁汤（PCN）对支气管哮喘气道炎症的保护作用，重点研究自噬及其潜在的分子机制。
采用人气道上皮细胞（16HBE）脂多糖（LPS）诱导的炎症损伤模型，评估 PCN 的作用。通过各种实验评估自噬相关基因、自噬体和囊泡计数以及活性氧（ROS）水平的表达。
首先，PCN 减轻了 LPS 诱导的细胞炎症。其次，PCN 减少了自噬体和自噬囊泡的数量。第三，PCN 显著降低了活性氧（ROS）水平。最重要的是，PCN 还下调了 LPS 诱导的高迁移率族蛋白 B1（HMGB1）、Beclin-1 和自噬相关基因 5（ATG5）的表达，同时增强了 B 细胞淋巴瘤 2（Bcl-2）的表达，进一步降低了 LC3II/I 比值。
PCN 通过抑制 ROS/HMGB1/Beclin-1 介导的细胞自噬的过度表达，减轻 16HBE 的炎症反应。因此，它可能成为治疗支气管哮喘的潜在药物。
