Institute of Medical Microbiology and Virology, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany.
Institute for Medical Microbiology and Virology, Klinikum Oldenburg, Oldenburg, Germany.
Eur J Clin Microbiol Infect Dis. 2023 Nov;42(11):1355-1363. doi: 10.1007/s10096-023-04666-x. Epub 2023 Oct 5.
This study aims to evaluate the performance of two latest generation matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems in routine laboratory settings, focusing on turnaround time (TAT), time to results (TTR), hands-on time, and identification rate.
We conducted a time and motion study on three workflow scenarios to simulate different laboratory settings. Overall, 618 bacterial isolates from a tertiary hospital's laboratory were processed using the VITEK MS PRIME (bioMérieux) and the MALDI Biotyper sirius (Bruker Daltonics) and their corresponding databases VITEK IVD Database 3.2 and MBT reference library 12.
The target preparation process showed no significant difference in TAT, but the Biotyper workflow had a shorter hands-on time by 3 to 6 min. In the measurement process, TTR was three to five times shorter for the Biotyper sirius while hands-on time was significantly shorter for VITEK MS PRIME (approximately 1.5 min per target). The identification rate without retesting was 97.9% for VITEK MS PRIME and 98.9% for Biotyper sirius. Both systems achieved 100% agreement at genus and 96.2% at species level.
Both systems exhibited excellent identification rates for routine bacterial isolates. Due to its high speed, the Biotyper sirius is suited for laboratories with high sample throughput and a workflow designed for processing larger batches. The VITEK MS PRIME, with its "load and go" system accommodating up to 16 targets, reduces hands-on time, making it a reasonable choice for laboratories with fewer identifications overall but a higher number of targets and a workflow designed for parallel processing on different workstations.
本研究旨在评估两种最新代基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统在常规实验室环境中的性能，重点关注周转时间（TAT）、出结果时间（TTR）、手动时间和鉴定率。
我们对三种工作流程场景进行了时间和运动学研究，以模拟不同的实验室环境。总体而言，使用 VITEK MS PRIME（生物梅里埃）和 MALDI Biotyper sirius（布鲁克·道尔顿）及其相应的数据库 VITEK IVD 数据库 3.2 和 MBT 参考库 12 对来自三级医院实验室的 618 株细菌分离株进行了处理。
目标制备过程在 TAT 方面没有显著差异，但 Biotyper 工作流程的手动时间缩短了 3 至 6 分钟。在测量过程中，Biotyper sirius 的 TTR 缩短了三到五倍，而 VITEK MS PRIME 的手动时间明显缩短（每个目标约 1.5 分钟）。无需重新测试的鉴定率为 VITEK MS PRIME 的 97.9%和 Biotyper sirius 的 98.9%。两个系统在属水平上的一致性达到 100%，在种水平上的一致性达到 96.2%。
两个系统对常规细菌分离株的鉴定率均表现出色。由于其速度快，Biotyper sirius 适用于具有高通量样本的实验室和设计用于处理更大批次的工作流程。VITEK MS PRIME 采用“加载即走”系统，最多可容纳 16 个目标，减少了手动时间，因此对于总体鉴定数量较少但目标数量较多且工作流程设计用于在不同工作站上并行处理的实验室来说是一个合理的选择。
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