Department of Periodontology, Faculty of Dentistry, Gelişim University, İstanbul, Turkey.
Department of Immunology, Aziz Sancar Institute of Experimental Medicine, İstanbul University, İstanbul, Turkey.
Niger J Clin Pract. 2023 Sep;26(9):1264-1272. doi: 10.4103/njcp.njcp_578_22.
Wound healing following periodontal soft tissue procedures can differ owing to different techniques, the feasibility of which can be determined through detailed macroscopic and microscopic observations.
This study aimed to clinically and histologically evaluate palatal wound healing in rats by secondary intention after excision using a steel scalpel, diode laser, and radiofrequency.
An excision was made in the edentulous anterior maxilla of 42 4-month-old male Wistar rats weighing 289-428 g. Part of the connective tissue was left in the surgical area to observe the dynamics of secondary intention wound healing. Three experimental groups were established: the steel scalpel, an 810-nm diode laser at a power output of 1.5 W in continuous mode, and a monopolar radiofrequency in a fully rectified waveform at 15 W. Clinical and histological analyses were performed on days 2, 4, and 7. Hemostasis, changes in body weight, defect size, epithelial gap, and inflammatory infiltration were evaluated.
The epithelial gap closed completely in all groups on day 7. Bleeding occurred significantly more in the scalpel group (P < 0.001). No significant changes were observed in body weight between the groups. Macroscopically, the mean wound area decreased over time in all groups. Wound healing was significantly slower in the laser group on day 2 and in the radiofrequency group on days 4 and 7 (P < 0.001). Microscopically, the laser created the cleanest wound area, with minimal inflammatory infiltration and no thermal injury. More damage occurred in the connective tissue of the radiofrequency group. Wound healing was observed on day 7 in all groups.
Palatal wound healing with secondary intention yielded different outcomes in a rat model when different techniques were used. However, almost complete healing was observed in all wounds, which highlights the importance of the soft tissue left in the surgical area. Wound healing in periodontal soft tissue procedures is not compromised by different techniques, as long as the clinician has sufficient knowledge and experience.
由于不同的技术，牙周软组织手术后的伤口愈合情况可能会有所不同，而这些技术的可行性可以通过详细的宏观和微观观察来确定。
本研究旨在通过手术刀、二极管激光和射频的二次意图切除，临床和组织学评估大鼠腭部伤口愈合。
在 42 只 4 月龄、体重 289-428g 的雄性 Wistar 大鼠无牙前上颌切除部分结缔组织，观察二次意图伤口愈合的动态。建立三个实验组：手术刀、功率输出为 1.5W 的 810nm 二极管激光连续模式和 15W 的单极射频全整流波形。在第 2、4 和 7 天进行临床和组织学分析。评估止血、体重变化、缺损大小、上皮间隙和炎症浸润。
所有组在第 7 天上皮间隙完全闭合。手术刀组出血明显更多（P<0.001）。组间体重无明显变化。所有组的平均伤口面积随时间减少。激光组第 2 天、射频组第 4 天和第 7 天愈合明显较慢（P<0.001）。显微镜下，激光组创造了最清洁的伤口区，炎症浸润最小，无热损伤。射频组的结缔组织损伤更大。所有组在第 7 天观察到伤口愈合。
在大鼠模型中，使用不同技术时，二次意图腭部伤口愈合会产生不同的结果。然而，所有伤口几乎完全愈合，这突出了手术区域留下的软组织的重要性。只要临床医生有足够的知识和经验，不同技术不会影响牙周软组织手术中的伤口愈合。
