Prognostic significance of copy number gains of detected by fluorescence hybridization in large B-cell lymphoma.

The protooncogene plays a critical role in many cellular processes. translocations are recurrent in large B-cell lymphomas (LBCLs) where they exhibit a negative effect on survival. Gain of copies is also frequently identified; however, there is no consensus on the frequency and prognostic significance of copy gains. We collected FISH data for with reflex testing for and and IHC results at diagnosis for a cohort of 396 and transformed LBCL cases and compared progression-free (PFS) and overall survival (OS) to determine the prognostic impact of extra copies. The prevalence of cases with copy number gain was 20.9%. PFS was shorter for patients with ≥5 copies compared to controls ( = 0.0005, HR = 2.25). gain trended towards worse OS; patients with ≥7 copies had worse OS ( = 0.013), similar to patients with translocations. We propose that gain represents a dose-dependent prognostic factor for LBCLs.