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RSPH6A在大鼠精子发生第一波及氧化应激条件下的表达：褪黑素的减弱作用

Expression of RSPH6A in the first wave of rat spermatogenesis and oxidative stress conditions: Attenuation by melatonin.

作者信息

Romano Maria Zelinda, Ben Rhouma Mariem, Messaoudi Imed, Aniello Francesco, Minucci Sergio, Venditti Massimo

机构信息

Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate "F. Bottazzi" Università degli Studi della Campania "Luigi Vanvitelli" Napoli Italy.

Laboratoire LR11ES41 Génétique Biodiversité et Valorisation des Bio-Ressourcés Institut Supérieur de Biotechnologie de Monastir, Université de Monastir Monastir Tunisia.

出版信息

Reprod Med Biol. 2023 Oct 2;22(1):e12542. doi: 10.1002/rmb2.12542. eCollection 2023 Jan-Dec.

DOI:10.1002/rmb2.12542
PMID:37795044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10545975/
Abstract

PURPOSE

Here, we report, for the first time, the temporal expression and localization of axonemal radial spoke head homolog A (RSPH6A) protein during the first wave of rat spermatogenesis and in oxidative stress conditions.

METHODS

For the developmental study, testes were collected from rats at different developmental stages (7, 14, 21, 28, 35, 42, and 60 postnatal days); for , 24 rats were treated with cadmium and/or melatonin. From each sample, western blot (WB) and immunofluorescence (IF) analyses for RSPH6A were performed.

RESULTS

RSPH6A expression starts at 21 PND alongside the appearance of I spermatocytes (SPC) with a significant increase up to 60 PND. Data were confirmed by IF analysis, showing that RPSH6A expression is restricted to I and II SPC, spermatids, and mature sperm. experiments showed that the expression and localization of RSPH6A in the testis and epididymal spermatozoa of adult rats treated with cadmium were impaired. Interestingly, melatonin (an antioxidant), given together with Cd, can counteract its damaging effects.

CONCLUSIONS

All combined data confirm that RSPH6A contributes to the onset of fertility by acting on sperm motility, raising the possibility of using RSPH6A as a marker for normal fertility in the general population.

摘要

目的

在此，我们首次报告轴丝辐条头同源物A（RSPH6A）蛋白在大鼠精子发生第一波期间以及氧化应激条件下的表达时间和定位。

方法

在发育研究中，从处于不同发育阶段（出生后7、14、21、28、35、42和60天）的大鼠收集睾丸；对于 ，24只大鼠用镉和/或褪黑素进行处理。对每个样本进行针对RSPH6A的蛋白质印迹（WB）和免疫荧光（IF）分析。

结果

RSPH6A表达在出生后21天随着I型精母细胞（SPC）的出现而开始，直至出生后60天显著增加。IF分析证实了该数据，显示RPSH6A表达局限于I型和II型SPC、精子细胞和成熟精子。 实验表明，镉处理的成年大鼠睾丸和附睾精子中RSPH6A的表达和定位受损。有趣的是，与镉一起给予的褪黑素（一种抗氧化剂）可以抵消其破坏作用。

结论

所有综合数据证实，RSPH6A通过作用于精子活力对生育能力的开始有贡献，增加了将RSPH6A用作普通人群正常生育能力标志物的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/14770d7662c1/RMB2-22-e12542-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/7b8e203b53f8/RMB2-22-e12542-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/68313c5007cd/RMB2-22-e12542-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/c9dacb02fbd3/RMB2-22-e12542-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/14770d7662c1/RMB2-22-e12542-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/7b8e203b53f8/RMB2-22-e12542-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/68313c5007cd/RMB2-22-e12542-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/c9dacb02fbd3/RMB2-22-e12542-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ad8/10545975/14770d7662c1/RMB2-22-e12542-g001.jpg

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