Pasteur Institute of São Paulo, São Paulo, Brazil.
Institute of Agricultural and Forestry Defense of the State of Espirito Santo, São Paulo, Brazil.
Virus Genes. 2023 Dec;59(6):817-822. doi: 10.1007/s11262-023-02030-x. Epub 2023 Oct 5.
Rabies is worldwide zoonosis caused by Lyssavirus rabies (RABV) a RNA negative sense virus with low level of fidelity during replication cycle. Nucleoprotein of RABV is the most conserved between all five proteins of the virus and is the most used gene for phylogenetic and phylogeographic studies. Despite of rabies been very important in Public Health concern, it demands continuous prophylactic care for herbivores with economic interest, such as cattle and horses. The main transmitter of RABV for these animals in Brazil is the hematophagous bats Desmodus rotundus. The aim of this study was to determine the dispersion over time and space of RABV transmitted by D. rotundus. Samples of RABV from the State of São Paulo (SP), Southeast Brazil isolated from the central nervous system (CNS) of cattle, were submitted to RNA extraction, RT-PCR, sequencing and phylogeographic analyzes with BEAST (Bayesian Evolutionary Analysis Sampling Trees) v 2.5 software. Was possible to identify high rate of diversification in starts sublineages of RABV what are correlated with a behavior of D. rotundus, the main transmitter of rabies to cattle. This study also highlights the importance of continuous monitoring of genetic lineages of RABV in Brazil.
狂犬病是一种全球性的动物传染病，由狂犬病病毒（RABV）引起。该病毒是一种 RNA 负链病毒，在复制周期中保真度较低。RABV 的核蛋白在病毒的所有五个蛋白中最保守，是进行系统发育和系统地理学研究最常用的基因。尽管狂犬病对公共卫生非常重要，但它需要对具有经济利益的食草动物（如牛和马）进行持续的预防性护理。在巴西，传播给这些动物的 RABV 的主要媒介是吸血蝙蝠 Desmodus rotundus。本研究旨在确定由 D. rotundus 传播的 RABV 的时间和空间分布。从巴西东南部圣保罗州（SP）从牛中枢神经系统（CNS）分离的 RABV 样本，经过 RNA 提取、RT-PCR、测序和 BEAST（贝叶斯进化分析采样树）v 2.5 软件进行系统地理学分析。结果表明，RABV 的起始亚谱系发生了高度多样化，这与 D. rotundus 的行为有关，后者是狂犬病向牛传播的主要媒介。本研究还强调了在巴西对 RABV 的遗传谱系进行持续监测的重要性。
