Gewirtz H, Olsson R A, Most A S
Cardiovasc Res. 1986 Jul;20(7):504-11. doi: 10.1093/cvr/20.7.504.
To test the hypothesis that adenosine is required to mediate the sustained increase in myocardial flow evoked by isoproterenol haemodynamic indices, myocardial blood flow (microspheres), and regional myocardial oxygen consumption were measured in eight closed chest, sedated pigs at control, after isoproterenol (6.9(2.8) ng X kg-1 X min-1 (mean (SD)) infused into the left anterior descending coronary artery, repeat control, and after a simultaneous infusion of the same dose of isoproterenol and adenosine deaminase (10 U X kg-1 X min-1). Data were acquired at one and 10 minutes after each infusion and compared with control measurements. Heart rate was held constant by atrial pacing. Peak left ventricular dP/dt (mm Hg X s-1) increased significantly (control 2190(32) mean (SD)) at both one (2900(302)) and 10 minutes (3010(391)) of isoproterenol infusion alone. At one minute of simultaneous infusion there was no change (control 1970(447)) in left ventricular dP/dt (2290(521)), although dP/dt was significantly increased at 10 minutes of simultaneous infusion (2790(483)). Transmural flow (ml X min-1 X g-1) increased significantly (control 1.49(0.46)) in the distal left anterior descending zone at one (1.94(0.48)) and 10 minutes (2.07(0.27)) of isoproterenol infusion alone. In contrast, flow failed to increase (control 1.65(0.27)) during the first minute of simultaneous infusion (1.73(0.38)), although it did increase significantly by 10 minutes (1.91(0.21). Finally, although myocardial oxygen consumption (ml X min-1 X 100 g-1) increased significantly (control 16.4(4.7)) at both one (20.1(4.7)) and 10 minutes (19.4(3.6)) of isoproterenol infusion alone, it failed to increase (control 18.2(3.8)) at one (19.3(4.6)) and 10 minutes (19.1(3.8)) of simultaneous infusion.(ABSTRACT TRUNCATED AT 250 WORDS)
为验证异丙肾上腺素引起心肌血流持续增加需腺苷介导这一假说，对8只闭胸、镇静的猪进行实验，测量其血流动力学指标、心肌血流量（微球法）和局部心肌氧耗。在对照状态下、向左前降支冠状动脉输注异丙肾上腺素（6.9(2.8) ng·kg-1·min-1（均值(标准差)）后、重复对照以及同时输注相同剂量异丙肾上腺素和腺苷脱氨酶（10 U·kg-1·min-1）后进行测量。每次输注后1分钟和10分钟采集数据并与对照测量值比较。通过心房起搏使心率保持恒定。单独输注异丙肾上腺素1分钟（2900(302)）和10分钟（3010(391)）时，左心室dp/dt峰值（mmHg·s-1）显著增加（对照2190(32)均值(标准差)）。同时输注1分钟时左心室dp/dt无变化（对照1970(447)）（2290(521)），尽管同时输注10分钟时dp/dt显著增加（2790(483)）。单独输注异丙肾上腺素1分钟（1.94(0.48)）和10分钟（2.07(0.27)）时，左前降支远端区域的透壁血流（ml·min-1·g-1）显著增加（对照1.49(0.46)）。相比之下，同时输注第1分钟时血流未增加（对照1.65(0.27)）（1.73(0.38)），尽管10分钟时显著增加（1.91(0.21)）。最后，单独输注异丙肾上腺素1分钟（20.1(4.7)）和10分钟（19.4(3.6)）时心肌氧耗（ml·min-1·100g-1）显著增加（对照16.4(4.7)），而同时输注1分钟（19.3(4.6)）和10分钟（19.1(3.8)）时心肌氧耗未增加（对照18.2(3.8)）。（摘要截选至250词）
