Matsuno K, Fujii H, Kotani M
Cell Tissue Res. 1986;246(2):263-9. doi: 10.1007/BF00215888.
The splenic macrophages of rats and mice were studied by light and fluorescence microscopy to determine their phagocytotic uptake of carbon and neutral polysaccharide (Fic-F), and their lysosomal enzyme activities. In rats, the large macrophages of the marginal zone (MZ) showed a moderate to strong acid phosphatase activity, and took up most of the Fic-F, even though they showed a weak phagocytotic activity to carbon particles. Red-pulp macrophages, however, ingested a large quantity of carbon particles, and are considered to be the major scavengers in the rat spleen. In contrast, the MZ macrophages in the mouse spleen were the major scavengers and showed a vigorous uptake of both carbon and Fic-F. In rats, the marginal metallophils (MM), located at the outer border of the periarterial lymphatic sheath and boundary between the MZ bridging channel and surrounding tissue, ingested Fic-F, whereas those located around the follicular area did not. In mice, on the other hand, the MM never ingested Fic-F. Lightly carbon-ladened small cells were constantly seen in the MZ of both rats and mice. They showed little acid phosphatase activity and did not ingest Fic-F. They were also present in the blood circulation.
通过光学显微镜和荧光显微镜研究大鼠和小鼠的脾巨噬细胞，以确定它们对碳和中性多糖（Fic-F）的吞噬摄取情况以及它们的溶酶体酶活性。在大鼠中，边缘区（MZ）的大型巨噬细胞显示出中度至强酸性磷酸酶活性，并摄取了大部分Fic-F，尽管它们对碳颗粒的吞噬活性较弱。然而，红髓巨噬细胞摄取大量碳颗粒，被认为是大鼠脾脏中的主要清除细胞。相比之下，小鼠脾脏中的MZ巨噬细胞是主要清除细胞，对碳和Fic-F均有强烈摄取。在大鼠中，位于动脉周围淋巴鞘外缘以及MZ桥接通道与周围组织边界处的边缘金属嗜细胞摄取Fic-F，而位于滤泡区域周围的则不摄取。另一方面，在小鼠中，边缘金属嗜细胞从不摄取Fic-F。在大鼠和小鼠的MZ中经常可以看到轻度负载碳的小细胞。它们显示出很少的酸性磷酸酶活性，不摄取Fic-F。它们也存在于血液循环中。
