Division of Operative Dentistry, Department of Restorative Dentistry, Piracicaba Dental School, Campinas State University - UNICAMP, Avenida Limeira 901, Piracicaba, São Paulo, Brazil.
Division of Biochemistry, Department of Biosciences, Piracicaba Dental School, Campinas State University - UNICAMP, Piracicaba, São Paulo, Brazil.
Clin Oral Investig. 2023 Nov;27(11):6835-6845. doi: 10.1007/s00784-023-05298-3. Epub 2023 Oct 6.
The aim of the study was to evaluate the effect of the addition of 10% nanohydroxyapatite in an experimental resin infiltrant on color stability and mineral loss.
Bovine enamel blocks were randomized into five groups (n = 27/group): SE (sound enamel); ICL (initial caries lesion); I (Icon®); E (experimental infiltrant); EH (experimental infiltrant containing 10% nanohydroxyapatite). Color evaluation (n = 15) was performed and CIELab* values were obtained at points T (baseline), T (14 days immersed on coffee solution), and T (28 days immersed) and data were calculated ∆E, ∆W, ∆L*, ∆a*, and ∆b*. Cross-sectional microhardness (n = 12) was performed and lesion area (∆S) was calculated. Images were obtained with polarized light optical microscopy at 40 × magnification (n = 5).
In color stability results, there was significant difference between time (14 and 28 days); ICL demonstrated significant difference among treated groups in all measures (∆L*, ∆a*, ∆b*, ∆E, ∆W) regardless of time; I and E demonstrated similar behavior on those measures and EH differed from I in ∆L*. For ∆S, ICL group showed a significant difference compared to I and EH groups, but did not differ from E.
The nanohydroxyapatite incorporation suggested an effective mineral recovery on initial caries lesion in depth; however, it showed high color variation, such as Icon. In terms of ∆S, I and EH had lower mineral loss, suggesting a reinforcement on initial caries lesion.
Commercial and experimental infiltrants containing nanohydroxyapatite present low color stability and might reinforce mineral in initial caries lesion.
本研究旨在评估在实验性树脂渗透剂中添加 10%纳米羟磷灰石对颜色稳定性和矿物质损失的影响。
将牛牙釉质块随机分为五组（每组 n = 27）：SE（正常釉质）；ICL（初始龋损）；I（Icon®）；E（实验性渗透剂）；EH（含 10%纳米羟磷灰石的实验性渗透剂）。进行颜色评估（n = 15），在 T 点（基线）、T 点（14 天浸泡于咖啡溶液中）和 T 点（28 天浸泡）获得 CIELab值，并计算 ∆E、∆W、∆L、∆a和 ∆b。进行横截面显微硬度（n = 12），计算病变面积（∆S）。在 40×放大倍数下用偏光光学显微镜获得图像（n = 5）。
在颜色稳定性结果中，时间（14 天和 28 天）之间存在显著差异；ICL 在所有测量指标（∆L*、∆a*、∆b*、∆E、∆W）中均显示出治疗组之间的显著差异，而与时间无关；I 和 E 在这些指标上表现出相似的行为，EH 与 I 在 ∆L*上不同。对于 ∆S，ICL 组与 I 和 EH 组相比存在显著差异，但与 E 组无差异。
纳米羟磷灰石的掺入表明对深度初始龋损有有效的矿物质再矿化作用；然而，它表现出高颜色变化，如 Icon。就 ∆S 而言，I 和 EH 具有较低的矿物质损失，表明对初始龋损具有增强作用。
含纳米羟磷灰石的商业和实验性渗透剂颜色稳定性低，可能增强初始龋损的矿物质。
