Department of Clinical Laboratory, Shanghai Gongli Hospital, The Second Military Medical University,Shanghai 200135, People's Republic of
J Genet. 2023;102.
This study aimed to identify the potential circular RNAs (circRNAs) in exosomes isolated from serum as biomarkers of lower limb vascular disease (LLVD) in patients with type 2 diabetes mellitus (T2DM). This research collected circRNAs from exosomes isolated from three T2DM patients and three T2DM patients with LLVD for microarray analysis. Five candidate biomarkers derived from differentially expressed circRNAs were then validated by quantitative real-time polymerase chain reaction in 20 T2DM patients and 20 T2DM patients with LLVD. Finally, expression levels of circRNAs were validated in 160 samples. Significant differences in the expression of 295 circRNAs were found between T2DM controls and T2DM patients with LLVD. Among them, 191 differentially expressed circRNAs were upregulated, and 104 were downregulated in T2DM patients with LLVD. Three upregulated and two downregulated circRNAs were further confirmed in 40 samples. According to the testing of 160 samples, hsa_circ_0001842 showed a noticeable specificity in the T2DM patients with LLVD group ( = 80), with an area under the curve (AUC) of 0.79, a sensitivity of 88.75%, and a specificity of 68.75%. In conclusion, hsa_circ_0001842 was found as a potential diagnostic biomarker for T2DM with LLVD.
本研究旨在鉴定 2 型糖尿病（T2DM）患者下肢血管疾病（LLVD）血清外泌体中潜在的环状 RNA（circRNA）作为生物标志物。本研究通过微阵列分析，从 3 名 T2DM 患者和 3 名 T2DM 合并 LLVD 患者的外泌体中提取 circRNA。然后，通过定量实时聚合酶链反应在 20 名 T2DM 患者和 20 名 T2DM 合并 LLVD 患者中验证了 5 个差异表达 circRNA 的候选生物标志物。最后，在 160 个样本中验证了 circRNA 的表达水平。在 T2DM 对照组和 T2DM 合并 LLVD 患者之间发现了 295 个 circRNA 的表达存在显著差异。其中，191 个差异表达的 circRNA 在 T2DM 合并 LLVD 患者中上调，104 个下调。在 40 个样本中进一步验证了 3 个上调和 2 个下调的 circRNA。根据对 160 个样本的检测，hsa_circ_0001842 在 T2DM 合并 LLVD 组中表现出显著的特异性（ = 80），曲线下面积（AUC）为 0.79，灵敏度为 88.75%，特异性为 68.75%。总之，hsa_circ_0001842 被发现是 T2DM 合并 LLVD 的潜在诊断生物标志物。
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