School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China.
School of mathematics and Statistics, Shandong University, Weihai 264209, China.
Exp Biol Med (Maywood). 2022 Sep;247(18):1609-1618. doi: 10.1177/15353702221110648. Epub 2022 Jul 23.
Type 2 diabetes mellitus (T2DM) is a multifactorial disorder that leads to alterations in gene regulation. ncRNAs have the characteristics of tissue specificity, disease specificity, timing specificity, high stability and post transcriptional regulation effect. These preconditions are more conducive to promote ncRNA to become a new biomarker for clinical diagnosis. Our study aims to explore the relationship between circRNA, lncRNA, miRNA and T2DM, and to evaluate their diagnostic value for T2DM. A total of 101 pairs of T2DM and controls were conducted in the study. QRT-PCR was used to study the differential expression of circRNAs, miRNAs and lncRNAs. ROC curve was used to estimate their diagnostic value in T2DM. Compared with healthy controls, the expression levels of hsa_circ_0071106, hsa_circ_0000284, hsa_circ_0071271, hsa-miR-29a-5p, hsa-miR-3690, hsa-miR-607, lncRNA MEG3 and lncRNA TUG1were higher in T2DM (all < 0.05). The AUCs of hsa_circ_0071106, hsa-miR-607 and lncRNA TUG1 for diagnosis of T2DM were 0.563,0.645 and 0.642, respectively. The combined AUC of hsa-miR-607, lncRNA TUG1 and hsa_circ_0071106 was 0.798 ([0.720~0.875], < 0.001). Moreover, the sensitivity of combined diagnosis was 75.2% and the specificity was 100.0%. The levels of lncRNA TUG1, hsa-miR-607 and hsa_circ_0071106 in peripheral blood have potential clinical diagnostic value for T2DM.
2 型糖尿病（T2DM）是一种多因素疾病，导致基因调控改变。ncRNAs 具有组织特异性、疾病特异性、时间特异性、高稳定性和转录后调控作用等特点。这些前提条件更有利于促进 ncRNA 成为临床诊断的新生物标志物。本研究旨在探讨 circRNA、lncRNA、miRNA 与 T2DM 的关系，并评估其对 T2DM 的诊断价值。本研究共纳入 101 对 T2DM 患者和对照组。采用 QRT-PCR 研究 circRNAs、miRNAs 和 lncRNAs 的差异表达。ROC 曲线用于评估它们在 T2DM 中的诊断价值。与健康对照组相比，T2DM 患者 hsa_circ_0071106、hsa_circ_0000284、hsa_circ_0071271、hsa-miR-29a-5p、hsa-miR-3690、hsa-miR-607、lncRNA MEG3 和 lncRNA TUG1 的表达水平更高（均<0.05）。hsa_circ_0071106、hsa-miR-607 和 lncRNA TUG1 诊断 T2DM 的 AUC 分别为 0.563、0.645 和 0.642。hsa-miR-607、lncRNA TUG1 和 hsa_circ_0071106 的联合 AUC 为 0.798（[0.720~0.875]，<0.001）。此外，联合诊断的敏感性为 75.2%，特异性为 100.0%。外周血 lncRNA TUG1、hsa-miR-607 和 hsa_circ_0071106 水平对 T2DM 具有潜在的临床诊断价值。
