Boyle J M, Margison G P, Saffhill R
Carcinogenesis. 1986 Dec;7(12):1987-90. doi: 10.1093/carcin/7.12.1987.
The persistence of O6-n-butyldeoxyguanosine (O6-nBudG) in DNA, the presence of O6-alkylguanine DNA alkyltransferase (AT) activity in cell extracts, and cell survival following exposure to N-n-butyl-N-nitrosourea (BNU), have been measured in normal and xeroderma pigmentosum cell strains, both transformed and untransformed. The rates of removal of O6-nBudG did not correlate with AT activity but did correlate with the ability of strains to excise bulky DNA lesions. BNU and N-methyl-N-nitrosourea dose-response curves for cell killing suggests that both AT and excision may be involved in the repair of cytotoxic lesions.
在正常细胞株和着色性干皮病细胞株（包括转化的和未转化的）中，已对DNA中O6-正丁基脱氧鸟苷（O6-nBudG）的持久性、细胞提取物中O6-烷基鸟嘌呤DNA烷基转移酶（AT）的活性以及暴露于N-正丁基-N-亚硝基脲（BNU）后的细胞存活率进行了测定。O6-nBudG的去除速率与AT活性无关，但与各细胞株切除大片段DNA损伤的能力相关。细胞杀伤的BNU和N-甲基-N-亚硝基脲剂量反应曲线表明，AT和切除作用可能都参与了细胞毒性损伤的修复。
