Sugiyama Haruka, Katsuma Susumu
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
MicroPubl Biol. 2023 Sep 15;2023. doi: 10.17912/micropub.biology.000953. eCollection 2023.
The BmN-4 cell line originates from the ovaries of silkworm, , and possesses endogenous small interfering RNA (siRNA) and PIWI-interacting RNA (piRNA) pathways. BmN-4 cells are latently infected with (BmLV), an RNA virus whose replication is strictly controlled by both siRNA and piRNA pathways. Knockdown or knockout of the core factors of these two small RNA pathways increases BmLV RNA amount, which in turn inhibits cell growth. Here, we used the known RNAi suppressor CrPV-1A to assess whether the BmN-4 cell line can be used for screening the suppressors of siRNA and piRNA pathways.
BmN - 4细胞系源自家蚕卵巢，具有内源性小干扰RNA（siRNA）和PIWI相互作用RNA（piRNA）途径。BmN - 4细胞被一种RNA病毒家蚕浓核病毒（BmLV）潜伏感染，该病毒的复制受到siRNA和piRNA途径的严格控制。这两条小RNA途径核心因子的敲低或敲除会增加BmLV RNA的量，进而抑制细胞生长。在此，我们使用已知的RNA干扰抑制因子CrPV - 1A来评估BmN - 4细胞系是否可用于筛选siRNA和piRNA途径的抑制因子。
