载体酵母核糖核酸在逆转录环介导等温扩增法检测新型冠状病毒中的作用
Effect of carrier yeast RNAs in the detection of SARS-CoV-2 by RT-LAMP.
作者信息
Morais-Armas Samantha, Medina-Suárez Sara, Machín Félix
机构信息
Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Canary Islands, Spain.
Instituto de Tecnologías Biomédicas, Universidad de La Laguna, San Cristóbal de La Laguna, Canary Islands, Spain.
出版信息
MicroPubl Biol. 2023 Sep 20;2023. doi: 10.17912/micropub.biology.000979. eCollection 2023.
The COVID-19 pandemic caused by SARS-CoV-2 has underscored the need for rapid and accurate diagnostic methods. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) has emerged as a promising molecular tool in least developed countries due to its simplicity, speed, and sensitivity. Nevertheless, reliable SARS-CoV-2 detection can be challenged by the chain custody of the samples. In this context, carrier RNA can act as a preservative. In this study, we explored the potential of yeast total and transference RNA (tRNA) in the SARS-CoV-2 RT-LAMP. We have found that most optimal conditions are reached with 1 μg/μL tRNA in the RT-LAMP reaction.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的2019冠状病毒病大流行凸显了对快速准确诊断方法的需求。逆转录环介导等温扩增技术（RT-LAMP）因其简单、快速和灵敏，已成为最不发达国家一种很有前景的分子工具。然而，样本的链条保管可能会对可靠检测SARS-CoV-2构成挑战。在这种情况下，载体RNA可以起到防腐剂的作用。在本研究中，我们探索了酵母总RNA和转移RNA（tRNA）在SARS-CoV-2 RT-LAMP中的潜力。我们发现，在RT-LAMP反应中加入1μg/μL的tRNA可达到最佳条件。
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