利用表面增强拉曼光谱分析严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突受体结合域（RBD）与血管紧张素转换酶2（ACE2）的结合及其受真菌共凝集素C的抑制作用。

Wetzel Christoph, Jansen-Olliges Linda, Stadler Marc, Surup Frank, Zeilinger Carsten, Roth Bernhard

Leibniz University Hannover, Hannover Centre for Optical Technologies, Nienburger Str. 17, 30167 Hannover, Germany.

Leibniz University Hannover, Centre of Biomolecular Drug Research, Schneiderberg 38, 30167 Hannover, Germany.

Biomed Opt Express. 2023 Jul 12;14(8):4097-4111. doi: 10.1364/BOE.495685. eCollection 2023 Aug 1.

The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.

利用表面增强拉曼光谱分析了严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突受体结合域（RBD）和人类血管紧张素转换酶2（ACE2）的结构以及由于结合活性导致的结构变化。应用抑制剂共海鞘素C来抑制刺突RBD与ACE2之间的结合。通过酰胺带的去卷积和主成分分析确定拉曼光谱的差异和变化。因此，我们展示了一种对蛋白质结构进行快速且无标记的分析方法，以及区分结合态和未结合态的方法。该方法适用于传感和筛选，也可能与研究其他蛋白质系统相关。