Yao Yuanzhi, Wang Jinhong, Wang Ziqi, Li Shenghua, Tan Hongliang
Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China.
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China.
Anal Methods. 2023 Oct 19;15(40):5351-5359. doi: 10.1039/d3ay01425h.
The detection of carcinoembryonic antigen (CEA) has profound implications in cancer diagnostics and therapeutic monitoring. In this work, we developed a colorimetric immunoassay for the detection of CEA. This assay involves the utilization of zinc(II)-based coordination polymers (ZnCPs) as a host for integrating glucose oxidase (GOx) and anti-carcinoembryonic antigen antibody (anti-CEA), which results in the formation of a detection antibody (anti-CEA/GOx@ZnCPs). The adaptable inclusion properties of ZnCPs enable the preservation of the original catalytic behavior of GOx and antigen capture ability of anti-CEA. Consequently, the anti-CEA/GOx@ZnCPs can act as a detection antibody to facilitate the development of an immunoassay. The combination of anti-CEA/GOx@ZnCPs in the immunoassay triggers a cascade reaction involving GOx and MnO nanosheets, leading to the generation of an amplified colorimetric signal through self-supplying oxygen. This colorimetric immunoassay exhibits a linear response ranging from 2 to 180 ng mL CEA and has a detection limit of 50 pg mL. The practicality of this colorimetric immunoassay in biological matrices was demonstrated by the successful determination of CEA in serum samples with good recovery and precision. We believe that this study will pave the way to rationally design multifunctional CP-based composites for a wide range of applications in bioanalysis.
癌胚抗原（CEA）的检测在癌症诊断和治疗监测中具有深远意义。在这项工作中，我们开发了一种用于检测CEA的比色免疫分析方法。该分析方法利用锌（II）基配位聚合物（ZnCPs）作为宿主，用于整合葡萄糖氧化酶（GOx）和抗癌胚抗原抗体（抗CEA），从而形成检测抗体（抗CEA/GOx@ZnCPs）。ZnCPs具有适应性的包合特性，能够保留GOx的原始催化行为和抗CEA的抗原捕获能力。因此，抗CEA/GOx@ZnCPs可以作为检测抗体，促进免疫分析方法的开发。在免疫分析中，抗CEA/GOx@ZnCPs的组合引发了涉及GOx和MnO纳米片的级联反应，通过自供氧产生放大的比色信号。这种比色免疫分析方法对CEA的线性响应范围为2至180 ng mL，检测限为50 pg mL。通过成功测定血清样品中的CEA并具有良好的回收率和精密度，证明了这种比色免疫分析方法在生物基质中的实用性。我们相信，这项研究将为合理设计基于配位聚合物的多功能复合材料在生物分析中的广泛应用铺平道路。
