Department of Biology, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
Institute of Graduate Studies in Health Science, Istanbul University, Istanbul, Turkey.
Ann Hematol. 2024 Apr;103(4):1035-1047. doi: 10.1007/s00277-023-05448-3. Epub 2023 Oct 6.
After hematopoietic stem cell transplantation, chimerism assay is a useful approach to monitor the success of the transplant and to select the appropriate treatment strategy, such as donor leukocyte infusion or immunosuppressive drug dosage. Short tandem repeat PCR is the method that has been accepted as the gold standard for chimerism. However, it has not yet been sufficient to detect mixed chimerism in patients with minimal residual disease. Simultaneously, recent years have been marked by developing sensitive, high-throughput, and accurate molecular genetic assays. These novel methods have subsequently been adapted for the analysis of post-transplant chimerism. In this review, we discuss the technical features of both novel and conventional gold standard chimerism assays. We also discuss their advantages and disadvantages.
造血干细胞移植后，嵌合状态检测是一种监测移植成功并选择合适治疗策略的有效方法，如供者白细胞输注或免疫抑制药物剂量。短串联重复序列 PCR 已被认为是嵌合状态检测的金标准方法。然而，在检测微小残留病患者的混合嵌合状态方面，它还不够充分。同时，近年来出现了一些敏感、高通量和准确的分子遗传学检测方法。这些新方法随后被用于移植后嵌合状态的分析。在这篇综述中，我们讨论了新型和传统金标准嵌合状态检测方法的技术特点。我们还讨论了它们的优缺点。
