Engineering Research Center of Fungal Biotechnology, Anhui Agricultural University, Hefei 230036, China.
Natural Products Laboratory, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui Province 230036, China.
J Pharm Biomed Anal. 2024 Jan 5;237:115749. doi: 10.1016/j.jpba.2023.115749. Epub 2023 Sep 23.
Cordyceps chanhua on artificial media has been approved as a medicine food homology product. However, the metabolites have not been extensively studied. HPLC-HRMS analysis showed that there were 11 main metabolites in the EtOAc extract including 4 probable unknown compounds. Fumosoroseain A with anti-aging effects is the most abundant known compound and was identified from C. chanhua for the first time. The second abundant compound is N-(2-Hydroxyethyl) adenosine, a typical metabolite of C. chanhua. All the known compounds have consistent health function with that of the fungus. HRMS, 1D and 2D NMR analyses revealed that compounds 2, 3, and 4 are new nucleosides named as chanhuanosides A, B, and C. Compound 1 is the known compound cordyrrole B isolated from C. chanhua for the first time whose structure is firstly confirmed by single crystal X-ray analysis. Bioactivity analysis revealed that 1-4 significantly inhibited pancreatic lipase activity, and strongly promoted the proliferation of RAW264.7 and 293T cells, suggesting that they might have ant-obesity, immunoregulation, and renal protection functions. Structure-bioactivity analysis revealed that the esterification on ribose can increase their bioactivity. Present metabolites study suggests that C. chanhua cultured on the artificial medium is a promising health food.
蝉花人工培养物已被批准为食药同源产品。然而，其代谢产物尚未得到广泛研究。HPLC-HRMS 分析表明，在 EtOAc 提取物中存在 11 种主要代谢产物，包括 4 种可能的未知化合物。具有抗衰老作用的福莫罗司因 A 是最丰富的已知化合物，这是首次从蝉花中鉴定出该化合物。第二丰富的化合物是 N-(2-羟乙基)腺苷，这是蝉花的一种典型代谢产物。所有已知化合物都具有与真菌相同的健康功能。高分辨质谱、1D 和 2D NMR 分析表明，化合物 2、3 和 4 是新的核苷，分别命名为蝉花苷 A、B 和 C。化合物 1 是首次从蝉花中分离得到的已知化合物 cordyrrole B，其结构首次通过单晶 X 射线分析得到确认。生物活性分析表明，化合物 1-4 能显著抑制胰脂肪酶的活性，并能强烈促进 RAW264.7 和 293T 细胞的增殖，提示它们可能具有抗肥胖、免疫调节和肾脏保护作用。结构-生物活性分析表明，核糖的酯化可以提高它们的生物活性。目前的代谢物研究表明，在人工培养基中培养的蝉花是一种很有前途的保健品。
