Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, "the 14th Five-Year Plan" Application Characteristic Discipline of Hunan Province (Clinical Medicine), Changsha Medical University, Changsha, 410219, PR China; Wuzhou Medical College, Wuzhou, 543100, PR China.
Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Functional Nucleic Acid, Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, "the 14th Five-Year Plan" Application Characteristic Discipline of Hunan Province (Clinical Medicine), Changsha Medical University, Changsha, 410219, PR China.
Talanta. 2024 Jan 15;267:125261. doi: 10.1016/j.talanta.2023.125261. Epub 2023 Oct 1.
Peptides with highly sequence-dependent recognition, assembly, and encoding abilities can perform functions similar to DNA or even better, such as biosensing, molecular information processing, coding, or storage. However, the combination of versatile peptides and 2D materials are rarely used for multipurpose integrated applications, including biosensing, information processing and security. Herein, peptide-graphene sensing system was comprehensively used for dual-signal sensing of tumor-derived exosomes (TDEs), logic computing, and information protection. The system used fluorescent-labeled CD63-binding peptide CP05 and graphene oxide (GO) to selectively detect CD63 and TDEs by fluorescence and resonance light scattering. From three levels such as matter, energy, and information analysis, the matter and energy changes in GO-CP05 peptide sensing system were transformed into valuable information, which achieve the dual-mode quantitative detection of TDEs and its marker CD63, and the actual serum analysis. This matter-energy interaction network was also informationized, and utilized for parallel and batch logic computing, two kinds of molecular crypto-steganography (based on peptide sequence and Boolean logic relationships), which facilitates development of intelligent sensing and advanced information technology. This work not only provides a new method for sensitive detection of important disease markers, but also provides ideas for integrating molecular sensing and informatization to open molecular digitization.
具有高度序列依赖性识别、组装和编码能力的肽可以执行类似于 DNA 的功能，甚至更好，例如生物传感、分子信息处理、编码或存储。然而，多功能肽与 2D 材料的组合很少用于多功能集成应用，包括生物传感、信息处理和安全。在此，全面利用肽-石墨烯传感系统进行肿瘤衍生外泌体（TDEs）的双信号传感、逻辑计算和信息保护。该系统使用荧光标记的 CD63 结合肽 CP05 和氧化石墨烯（GO）通过荧光和共振光散射来选择性地检测 CD63 和 TDEs。从物质、能量和信息分析三个层面，将 GO-CP05 肽传感系统中的物质和能量变化转化为有价值的信息，实现 TDEs 及其标志物 CD63 的双模式定量检测，以及实际血清分析。这个物质-能量相互作用网络也实现了信息化，并用于并行和批量逻辑计算、两种分子隐写术（基于肽序列和布尔逻辑关系），这有利于智能传感和先进信息技术的发展。这项工作不仅为重要疾病标志物的灵敏检测提供了新方法，也为分子传感和信息化的集成提供了思路，开辟了分子数字化的新途径。
