基于金纳米粒子二聚体的免疫层析法原位超灵敏检测猪流行性腹泻病毒。
Gold nanoparticle dimer-based immunochromatography for in situ ultrasensitive detection of porcine epidemic diarrhea virus.
机构信息
College of Animal Science and Technology, Guangxi University, Nanning, 530005, Guangxi, China.
Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530004, Guangxi, China.
出版信息
Mikrochim Acta. 2023 Oct 7;190(11):430. doi: 10.1007/s00604-023-06013-5.
The low detection sensitivity of lateral-flow immunochromatography assay (LFIA) based on spherical gold nanoparticle (AuNP) limits its wide applications. In the present study, AuNP dimers with strong plasma scattering and robust signal output were synthesized via the Ag ion soldering (AIS) strategy and used as labeled probes in LFIA to boost the sensitivity without any extra operation process and equipment. The established LFIA exhibited high sensitivity with a limit of detection (LOD) of 2.0 × 10 TCID/mL for PEDV, which provides 50 times higher sensitivity than commercial LFIA based on spherical colloidal gold. In addition, the AuNP dimer-based LFIA showed strong specificity, good reproducibility, high stability, and good accordance to reverse transcription polymer chain reaction (RT-PCR) when detecting 109 clinical samples. Thus, the AuNP dimers is a promising probe for LFIA and the developed AuNP dimer-based LFIA is suitable for the rapid detection of PEDV in the field.
基于球形金纳米粒子（AuNP）的侧向流动免疫层析分析（LFIA）的低检测灵敏度限制了其广泛应用。在本研究中，通过银离子焊接（AIS）策略合成了具有强等离子体散射和稳健信号输出的 AuNP 二聚体，并将其用作 LFIA 中的标记探针，以在不增加任何额外操作过程和设备的情况下提高灵敏度。所建立的 LFIA 表现出高灵敏度，PEDV 的检测限（LOD）为 2.0×10 TCID/mL，比基于球形胶体金的商业 LFIA 高 50 倍。此外，当检测 109 个临床样本时，基于 AuNP 二聚体的 LFIA 表现出强特异性、良好的重现性、高稳定性和与逆转录聚合酶链反应（RT-PCR）的良好一致性。因此，AuNP 二聚体是 LFIA 的一种有前途的探针，所开发的基于 AuNP 二聚体的 LFIA 适用于现场快速检测 PEDV。
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