School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan, 450001, PR China.
School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan, 450001, PR China.
Talanta. 2024 Jan 15;267:125257. doi: 10.1016/j.talanta.2023.125257. Epub 2023 Sep 30.
In this work, a novel ultrasensitive aptasensor for deoxynivalenol (DON) detection based on the polyethyleneimine-functionalised porous reduced graphene oxide loaded gold nanowires (PEI-PrGO/AuNWs) and methylene blue (MB)-labelled zeolitic imidazolate framework-8 (ZIF-8) signal amplification strategy was proposed. PEI-PrGO/AuNWs with large surface area and excellent conductivity were used as modification materials on bare gold electrodes, which could increase the combining of complementary strand (cDNA) on the electrode substrate and accelerate the electron transfer efficiency. Furthermore, a novel electrochemical signal probe was synthesized using streptavidin-modified zeolitic imidazolate framework-8 (ZIF-8/SA) as a carrier loaded with MB and reverse complementary chain (sDNA). In the presence of DON, the signal probe was introduced to the electrode surface by Watson-Crick base pairing after specific binding of DON to the aptamer (Apt). As expected, under the optimal conditions, the DON concentration was linearly related to the peak current generated by the prepared aptasensor, and the measured data were combined with theoretical calculations to obtain a detection limit of 2.23 × 10 mg/mL.
在这项工作中，提出了一种基于聚乙烯亚胺功能化多孔还原氧化石墨烯负载金纳米线（PEI-PrGO/AuNWs）和亚甲基蓝（MB）标记的沸石咪唑酯骨架-8（ZIF-8）信号放大策略的新型脱氧雪腐镰刀菌烯醇（DON）检测超灵敏适体传感器。PEI-PrGO/AuNWs 具有大的表面积和优异的导电性，被用作裸金电极上的修饰材料，这可以增加互补链（cDNA）在电极基底上的结合，并加速电子转移效率。此外，使用链霉亲和素修饰的沸石咪唑酯骨架-8（ZIF-8/SA）作为载体，负载 MB 和反向互补链（sDNA），合成了一种新型电化学信号探针。在存在 DON 的情况下，DON 特异性结合适体（Apt）后，通过 Watson-Crick 碱基配对将信号探针引入电极表面。正如预期的那样，在最佳条件下，DON 浓度与制备的适体传感器产生的峰电流呈线性关系，并且将测量数据与理论计算相结合，得到检测限为 2.23×10 mg/mL。
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