Chemistry Department of Lomonosov Moscow State University, Moscow 119991, Russia.
All-Russian Research Institute of Phytopathology, Bolshiye Vyazemy 143050, Russia.
Int J Mol Sci. 2024 Sep 2;25(17):9534. doi: 10.3390/ijms25179534.
The quality of food is one of the emergent points worldwide. Many microorganisms produce toxins that are harmful for human and animal health. In particular, mycotoxins from Fusarium fungi are strictly controlled in cereals. Simple and robust biosensors are necessary for 'in field' control of the crops and processed products. Nucleic acid-based sensors (aptasensors) offer a new era of point-of-care devices with excellent stability and limits of detection for a variety of analytes. Here we report the development of a surface-enhanced Raman spectroscopy (SERS)-based aptasensor for the detection of T-2 and deoxynivalenol in wheat grains. The aptasensor was able to detect as low as 0.17% of pathogen fungi in the wheat grains. The portable devices, inexpensive SERS substrate, and short analysis time encourage further implementation of the aptasensors outside of highly equipped laboratories.
食品质量是全球关注的焦点之一。许多微生物会产生毒素，这些毒素对人类和动物的健康有害。特别是，真菌毒素（Fusarium fungi）在谷物中受到严格控制。简单而强大的生物传感器对于农作物和加工产品的“现场”控制是必要的。基于核酸的传感器（适配体传感器）为各种分析物提供了具有出色稳定性和检测限的即时检测设备的新时代。在这里，我们报告了一种基于表面增强拉曼光谱（SERS）的适配体传感器的开发，用于检测小麦中的 T-2 和脱氧雪腐镰刀菌烯醇。该适配体传感器能够检测到小麦中低至 0.17%的病原菌真菌。便携式设备、廉价的 SERS 基底和短的分析时间鼓励在没有高度配备的实验室的情况下进一步实施适配体传感器。
