Department of Analytical Chemistry and Pollutants, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran.
Department of Analytical Chemistry and Pollutants, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran.
Talanta. 2024 Jan 15;267:125241. doi: 10.1016/j.talanta.2023.125241. Epub 2023 Oct 1.
Today, the widespread use of opioid and analgesic drugs (OAs) has caused global concern due to their addictive properties and side effects. Therefore, in this study, polyvinyl alcohol (PVA)/poly acrylic acid (PAA)/MOF NiCoZn-LDH@graphene oxide (GO) electrospun nanofiber was synthesized and employed as an effective and novel sorbent at thin-film microextraction (TF-μSPE) method for the fast and simultaneous extraction of seven opioid and analgesic drugs in human biological fluids (plasma, urine) before performing quantitative analysis by high-pressure liquid chromatography (HPLC-UV) device. This new nano-absorbent was characterized by energy dispersive X-ray spectrometer (EDX), X-ray photoelectron spectroscope (XPS), Fourier transforms infrared spectrometer (FT-IR), field emission scanning electron microscopy (FE-SEM), X-ray diffraction analysis (XRD), and nitrogen absorption-desorption analysis (BET). The combination of MOF NiCoZn-LDH@GO with a highly porous structure and rich functional groups in the PVA/PAA substrate casing significantly improves the absorption properties of the nanofibers. In other words, the existence, of MOF NiCoZn-LDH@GO composite in the polymer network PVA/PAA causes an increase in the extraction efficiency of the electrospinning adsorbent due to the creation of hydrogen bonds and π-π interactions with the intended analytes. Various effective factors in the extraction efficiency of the desired analytes were optimized using a one-variable-at-a-time method. Under the optimum conditions, the linearity dynamic range was achieved in the range of 0.3-1000.0 for caffeine, naloxone, noscapine, and celecoxib, and 0.5-1000.0 μg L for tramadol, codeine, and hydrocodone with correlation coefficients ≥0.999. The lowest detection limit (LODs) and the lowest quantitative limit (LOQs) of the TF-μSPE method were obtained in the range of (0.1-0.15) and (0.3-0.5), respectively.
今天，由于阿片类药物和镇痛药（OAs）具有成瘾性和副作用，其广泛使用引起了全球关注。因此，在这项研究中，合成了聚乙烯醇（PVA）/聚丙烯酸（PAA）/MOF NiCoZn-LDH@氧化石墨烯（GO）电纺纳米纤维，并将其作为一种有效的新型吸附剂，用于在高效液相色谱（HPLC-UV）装置进行定量分析之前，通过薄膜微萃取（TF-μSPE）方法快速同时提取人生物体液（血浆、尿液）中的七种阿片类药物和镇痛药。这种新型纳米吸收剂的特点是能量色散 X 射线光谱仪（EDX）、X 射线光电子能谱仪（XPS）、傅里叶变换红外光谱仪（FT-IR）、场发射扫描电子显微镜（FE-SEM）、X 射线衍射分析（XRD）和氮气吸收-解吸分析（BET）。MOF NiCoZn-LDH@GO 与高度多孔结构和丰富的官能团在 PVA/PAA 基质外壳中的结合，显著提高了纳米纤维的吸附性能。换句话说，MOF NiCoZn-LDH@GO 复合材料在聚合物网络 PVA/PAA 中的存在，由于与目标分析物形成氢键和π-π相互作用，导致电纺吸附剂的萃取效率提高。使用单变量法优化了目标分析物萃取效率的各种有效因素。在最佳条件下，咖啡因、纳洛酮、那可丁和塞来昔布的线性动态范围为 0.3-1000.0μg/L，曲马多、可待因和氢可酮的线性动态范围为 0.5-1000.0μg/L，相关系数≥0.999。薄膜微萃取（TF-μSPE）方法的最低检测限（LOD）和最低定量限（LOQ）分别在（0.1-0.15）和（0.3-0.5）范围内。
