Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Talanta. 2024 Mar 1;269:125403. doi: 10.1016/j.talanta.2023.125403. Epub 2023 Nov 10.
In the present work, the rod-like ZIF-8 (ZIF8@E coli) was prepared by fast, easy and environmentally friendly method of biomimetic mineralization with Escherichia coli bacteria as a bio-template and was exploited for the first time in the microextraction. In this regard, electrospun nanofiber mats of polyacrylonitrile (PAN) and ZIF8@E coli were prepared by electrospinning method and used as a new sorbent for thin film microextraction (TFME) of benzoylurea insecticides such as Hexaflumuron and Teflubenzuron as model analytes. The PAN/ZIF8@E coli nanocomposite was characterized using electron scanning microscopy and various spectroscopy techniques. Factors affecting the proposed extraction method were screened and optimized using the experiment design strategy. Then, the model analytes were measured by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detector after microextraction. Satisfactory figures of merit were obtained for suggested TFME-HPLC-UV under optimum conditions. The suitable linearity varied in the range of 0.5-200 μg L with R greater than 0.9968. The limit of detections for Hexaflumuron and Teflubenzuron were 0.12 and 0.15 μg L, respectively. The application of the method in the real sample was investigated by analyzing the selected analytes in environmental water and food samples. The spiking recovery of the selected analytes varied in the range of 93.0-109.8 % (RSD≤7.68). The results confirm the efficient application of this new sorbent in TFME approach. Considering the high availability, ease of production, and environmental friendliness of bacteria along with the significant improvement of metal-organic framework (MOF) growth efficiency, biomimetic mineralization is expected to be efficient method for the synthesis of ordered MOFs for use in extraction fields.
在本工作中，通过快速、简便且环保的仿生矿化方法，以大肠杆菌细菌为生物模板制备了棒状 ZIF-8（ZIF8@E coli），并首次将其应用于微萃取中。在这方面，通过静电纺丝方法制备了聚丙烯腈（PAN）和 ZIF8@E coli 的电纺纳米纤维垫，并将其用作新型的薄膜微萃取（TFME）的吸附剂，用于萃取Hexaflumuron 和 Teflubenzuron 等苯甲酰脲类杀虫剂作为模型分析物。使用电子扫描显微镜和各种光谱技术对 PAN/ZIF8@E coli 纳米复合材料进行了表征。使用实验设计策略筛选和优化了影响所提出的萃取方法的因素。然后，在微萃取后通过高效液相色谱（HPLC）与紫外（UV）检测器对模型分析物进行测量。在最佳条件下，建议的 TFME-HPLC-UV 获得了令人满意的计量指标。合适的线性范围在 0.5-200μg/L 之间，相关系数大于 0.9968。Hexaflumuron 和 Teflubenzuron 的检测限分别为 0.12 和 0.15μg/L。通过分析环境水样和食品样品中的选定分析物，研究了该方法在实际样品中的应用。选定分析物的加标回收率在 93.0-109.8%（RSD≤7.68）之间。结果证实了这种新型吸附剂在 TFME 方法中的有效应用。考虑到细菌的高可用性、易于生产和环境友好性，以及金属有机骨架（MOF）生长效率的显著提高，仿生矿化有望成为用于萃取领域的有序 MOF 合成的有效方法。
