Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, Telangana, India.
Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, Telangana, India.
Pharmacol Biochem Behav. 2023 Nov;232:173652. doi: 10.1016/j.pbb.2023.173652. Epub 2023 Oct 5.
Obsessive-compulsive disorder (OCD) is a disabling mental condition that poses recurring bothersome intrusive thoughts, obsessions, and compulsions. Considering the positive impact of probiotics on neuropsychiatric disorders, herein, we investigated the effect of multistrain probiotic (Bifidobacterium lactis UBBLa-70, Bacillus coagulans Unique IS-2, Lactobacillus rhamnosus UBLR-58, Lactobacillus plantarum UBLP-40, Bifidobacterium infantis UBBI-01, Bifidobacterium breve UBBr-01, and glutamine) in the management of OCD-like phenotype in rats. Rats injected with quinpirole for 5 weeks showed an increased number of marble burying and self-grooming episodes. Quinpirole-injected animals also did less head dipping in the hole board test and avoided exploration of open spaces in the elevated-plus maze. These repetitive, compulsive, self-directed, and anxiety-like phenotypes were abolished after 8-week of multistrain probiotic treatment. The probiotic formulation also prevented the elevated mRNA expression of interleukin-6, tumor-necrosis factor-α, and C-reactive protein in the amygdala and dysregulated levels of 5-hydroxytryptamine, dopamine, and noradrenaline in the frontal cortex of quinpirole-injected rats. The level of brain-derived neurotrophic factor in the frontal cortex remained unaffected across the groups. The altered levels of goblet cells and crypt-to-villi ratio in quinpirole rats were prevented by multistrain probiotic treatment. The results of 16S-rRNA gene-sequencing of gut microbiota from feces contents revealed an elevation in the abundance of Allobaculum and Bifidobacterium species (specifically Bifidobacterium animalis), while the presence of Lactobacillus species (including Lactobacillus reuteri and Lactobacillus vaginalis) exhibited a decline in quinpirole-induced rats. These results imply that modifying the gut-brain axis may be a possible mechanism by which selective multistrain probiotic therapy prevents OCD-like behaviors.
强迫症（OCD）是一种使人丧失能力的精神疾病，其特征是反复出现烦扰性的强迫性思维、强迫观念和强迫行为。鉴于益生菌对神经精神疾病的积极影响，在此，我们研究了多菌株益生菌（乳双歧杆菌 UBBLa-70、凝结芽孢杆菌 Unique IS-2、鼠李糖乳杆菌 UBLR-58、植物乳杆菌 UBLP-40、婴儿双歧杆菌 UBBI-01、短双歧杆菌 UBBr-01 和谷氨酰胺）对大鼠 OCD 样表型的管理作用。用喹硫平注射 5 周的大鼠表现出更多的埋丸和自我梳理行为。在洞板测试中，喹硫平注射动物的头探入次数减少，并且避免探索高架十字迷宫的开阔区域。这些重复的、强迫性的、自我导向的和焦虑样的表型在接受 8 周多菌株益生菌治疗后消失。益生菌配方还防止了杏仁核中白细胞介素-6、肿瘤坏死因子-α和 C 反应蛋白的 mRNA 表达升高，并调节了喹硫平注射大鼠前额皮质中 5-羟色胺、多巴胺和去甲肾上腺素的失调水平。前额皮质中的脑源性神经营养因子水平在各组之间保持不变。多菌株益生菌治疗可防止喹硫平大鼠中脑源性神经营养因子水平的改变。粪便内容物中肠道微生物群 16S-rRNA 基因测序的结果显示，Allobaculum 和双歧杆菌属（特别是双歧杆菌动物亚种）的丰度升高，而 Lactobacillus 属（包括 Lactobacillus reuteri 和 Lactobacillus vaginalis）的丰度下降在喹硫平诱导的大鼠中。这些结果表明，修饰肠道-大脑轴可能是选择性多菌株益生菌治疗预防 OCD 样行为的一种可能机制。
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