Halterman S D, Murdoch W J
Endocrinology. 1986 Dec;119(6):2417-21. doi: 10.1210/endo-119-6-2417.
Ewes were treated systemically during the periovulatory period with the antihistamines diphenhydramine (H1-receptor antagonist) and(or) cimetidine (H2-receptor antagonist). None of the treatments prevented ovulation. However, the combination of drugs inhibited subsequent function of the corpus luteum (CL) as assessed by serum measurements of progesterone. Administration of antihistamines did not alter pituitary secretion of LH. Injection of small quantities of diphenhydramine and cimetidine into the antrum of the preovulatory follicle also caused aberrant luteal activity, but no blockade of ovulation. Intrafollicular injection of histamine reversed the inhibitory influence of antihistamines on the CL. Antihistamines suppressed preovulatory thecal edema and antral influx of plasma transudate containing LH. Thus, the effects of antihistamines appear to have been exerted at the follicular level. Function of matured CL was not compromised by peripheral administration of antihistamines. Actions of histamine within the preovulatory ovine follicle do not appear to be a prerequisite for ovulation, but apparently do have an impact on ensuing luteal function. We propose that a hyperemic reaction to histamine in preovulatory follicles enhances vascular filtration of LH. As a consequence, maximal permeation of the avascular granulosal layer of the follicle by LH leads to a normal luteinization response.
在围排卵期对母羊进行全身用药，使用抗组胺药苯海拉明（H1受体拮抗剂）和（或）西咪替丁（H2受体拮抗剂）。所有处理均未阻止排卵。然而，通过血清孕酮测定评估，药物组合抑制了随后黄体（CL）的功能。抗组胺药的给药并未改变垂体促黄体生成素（LH）的分泌。向排卵前卵泡的卵泡腔注射少量苯海拉明和西咪替丁也会导致黄体活动异常，但未阻断排卵。卵泡内注射组胺可逆转抗组胺药对黄体的抑制作用。抗组胺药抑制排卵前的卵泡膜水肿和含有LH的血浆渗出液的卵泡腔流入。因此，抗组胺药的作用似乎是在卵泡水平发挥的。外周给予抗组胺药不会损害成熟黄体的功能。排卵前绵羊卵泡内组胺的作用似乎不是排卵的先决条件，但显然会对随后的黄体功能产生影响。我们提出，排卵前卵泡对组胺的充血反应会增强LH的血管滤过。结果，LH对卵泡无血管颗粒层的最大渗透导致正常的黄体化反应。
