Caldwell N, Gardner R
Endocr Res. 1986;12(3):255-67. doi: 10.3109/07435808609035440.
These studies were initiated to examine the effects of indomethacin on uterine contractile responses to oxytocin, carbachol, bradykinin, angiotension II and isoproterenol. Isolated uterine horns used in this study were removed from immature (21 days old) ovariectomized Sprague-Dawley rats (40-50 gm). Pre-treatment of uterine horns with indomethacin (10(-3) M) for periods of 15,30,60,90, and 180 seconds resulted in maximal inhibition of oxytocin-induced contractions by 15 seconds. In an indomethacin dose-response study, oxytocin-induced contractions were completely eliminated by a dose of indomethacin of 10(-3) M. Utilizing doses of indomethacin (3.5 X 10(-4) and 5.0 X 10(-4) M) that significantly reduced oxytocin-induced contractions by 48 and 77% respectively, contractile responses induced by carbachol (10(-6) M), bradykinin (10(-6) M), and angiotension II (4 X 10(-11) M) were not significantly reduced. Relaxation of uterine smooth muscle by isoproterenol (10(-5) M) in the presence of carbachol (10(-5) M) was not altered by indomethacin pretreatment. Data from this study demonstrate that indomethacin selectively inhibits oxytocin-induced uterine contractions in the immature rat.
开展这些研究是为了检测吲哚美辛对子宫对催产素、卡巴胆碱、缓激肽、血管紧张素II和异丙肾上腺素的收缩反应的影响。本研究中使用的离体子宫角取自未成熟（21日龄）、卵巢切除的Sprague-Dawley大鼠（40-50克）。用吲哚美辛（10(-3) M）对子宫角进行15、30、60、90和180秒的预处理，15秒时可使催产素诱导的收缩受到最大抑制。在一项吲哚美辛剂量反应研究中，10(-3) M的吲哚美辛剂量可完全消除催产素诱导的收缩。使用分别能使催产素诱导的收缩显著降低48%和77%的吲哚美辛剂量（3.5×10(-4)和5.0×10(-4) M）时，卡巴胆碱（10(-6) M）、缓激肽（10(-6) M）和血管紧张素II（4×10(-11) M）诱导的收缩反应未显著降低。在存在卡巴胆碱（10(-5) M）的情况下，吲哚美辛预处理未改变异丙肾上腺素（10(-5) M）引起的子宫平滑肌松弛。本研究数据表明，吲哚美辛可选择性抑制未成熟大鼠中催产素诱导的子宫收缩。
