Hartschuh W, Grube D
Arch Dermatol Res. 1979 Jun 25;265(2):115-22. doi: 10.1007/BF00407875.
Merkel cells of sinus hair follicles of nude mice were investigated by fluorescence and electron microscopy following pretreatment with amine precursors (L-Dopa, L-5-Hydroxytryptophan) and monoaminoxidase-inhibitors (Marsilid, Harmaline). Neither in control animals nor in pretreated animals any evidence for an involvement of Merkel cells in monoamine metabolism could be found. Therefore, the hypothesis that Merkel cells might share the most constant cytochemical characteristics of the cells of the APUD series, i.e., to take up amine precursors, should definitely be left. Following Harmaline treatment, however, Merkel cells were found fluorescent; cytophotometric analysis of the fluorescence emission spectra of formaldehyde-treated tissue ascertained that this fluorescence was due merely to a specific Harmaline fluorescence. The significance of Harmaline uptake in Merkel cells, most probably in the Merkel cell granules, is discussed.
在用胺前体（L - 多巴、L - 5 - 羟色氨酸）和单胺氧化酶抑制剂（吗氯贝胺、骆驼蓬碱）预处理后，通过荧光显微镜和电子显微镜对裸鼠鼻窦毛囊的默克尔细胞进行了研究。在对照动物和预处理动物中，均未发现任何证据表明默克尔细胞参与单胺代谢。因此，关于默克尔细胞可能具有APUD系列细胞最恒定的细胞化学特征（即摄取胺前体）这一假说，肯定应予以摒弃。然而，在用骆驼蓬碱处理后，发现默克尔细胞有荧光；对甲醛处理组织的荧光发射光谱进行细胞光度分析确定，这种荧光仅仅是由于特定的骆驼蓬碱荧光所致。讨论了默克尔细胞摄取骆驼蓬碱的意义，最有可能是在默克尔细胞颗粒中的意义。
