Veterinary Clinical Biochemistry Service (SBCV), Department of Biochemistry and Molecular Biology, School of Veterinary, Autonomous University of Barcelona, Spain.
Ruminant Production Program, IRTA (Institut de Recerca i Tecnologies Agroalimentàries), Torre Marimon, Caldes de Montbui, Spain.
Res Vet Sci. 2023 Nov;164:105042. doi: 10.1016/j.rvsc.2023.105042. Epub 2023 Oct 5.
The concentration of calprotectin in feces is a well-studied marker of gastrointestinal inflammation in humans. However, little is known about fecal calprotectin in farm animals. In this work, we have validated an immunoturbidimetric method for fecal calprotectin (Bühlmann fCAL® turbo assay, Schönenbuch, Switzerland) in porcine and bovine fecal samples. Linearity was evaluated by serial dilution (R > 0.97 was obtained for both species). Accuracy was assessed by a recovery study, with results between 80 and 120% for low, medium, and high samples in both species. Intra- and inter-assay variability was <20%. Limit of detection was 6.4 μg/g in pig and 5.3 μg/g in cow. Limit of quantification was 13.4 μg/g (pig) and 11.1 μg/g (cow). Additionally, clinical validation has been included to evaluate the ability of the assay to detect inflammatory status in the intestine under different management conditions. In experiments with porcine, it was found that piglets treated with ZnO had lower concentrations of fecal calprotectin. In a second experiment in bovine, calves with diarrhea had higher concentration of fecal calprotectin. The Bühlmann fCAL® turbo assay is suitable for measurement of calprotectin in porcine and bovine fecal samples. Moreover, fecal calprotectin could be a good biomarker of intestinal inflammation in both species.
粪便中钙卫蛋白的浓度是研究人类胃肠道炎症的一个很好的标志物。然而，关于家畜粪便中的钙卫蛋白知之甚少。在这项工作中，我们已经验证了一种用于猪和牛粪便样品的免疫比浊法检测粪便钙卫蛋白（Bühlmann fCAL® turbo 测定法，瑞士 Schönenbuch）。通过系列稀释评估了线性（两种物种的 R>0.97）。通过回收研究评估了准确性，两种物种的低值、中值和高值样品的回收率均在 80%至 120%之间。批内和批间变异性均<20%。在猪中检测限为 6.4μg/g，在牛中检测限为 5.3μg/g。定量限在猪中为 13.4μg/g，在牛中为 11.1μg/g。此外，还包括临床验证，以评估该测定法在不同管理条件下检测肠道炎症状态的能力。在猪的实验中，发现用 ZnO 处理的仔猪粪便中钙卫蛋白浓度较低。在牛的第二项实验中，患有腹泻的犊牛粪便中钙卫蛋白浓度较高。Bühlmann fCAL® turbo 测定法适用于猪和牛粪便中钙卫蛋白的测定。此外，粪便钙卫蛋白可能是两种物种肠道炎症的良好生物标志物。
