Tang Xiufeng, Li Ye, Jin Ruihe, Yin Guoyu, Hou Lijun, Liu Min, Ju Feng, Han Ping
Key Laboratory of Geographic Information Science (Ministry of Education), School of Geographic Sciences, East China Normal University, Shanghai, 200241, China.
State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200241, China.
Chemosphere. 2023 Dec;344:140377. doi: 10.1016/j.chemosphere.2023.140377. Epub 2023 Oct 6.
Quantification of polycyclic aromatic hydrocarbons (PAHs) in contaminated soil and identification of potential PAH degraders are essential for comprehending their environmental fate and conducting bioremediation. However, the microbial population responsible for the breakdown of phenanthrene (PHE) in polluted soil environments is frequently disregarded. In this study, via DNA-stable-isotope probing (DNA-SIP), we found that soil microbiota likely plays a crucial part in the PHE degradation. The PHE removal rates were 98% and 99%, in C-PHE and C-PHE microcosmic incubations, respectively. CO was produced along with the degradation of C-PHE. According to the analysis of 16S rRNA gene, there was a relatively higher presence of unidentified bacteria in the 'heavy' DNA fractions treated with C-PHE. Genus of Enterobacteriales, Acidobacteria, Alphaproteobacteria, Paenibacillaceae, Flavobacteriia, Chloroflexi, Cyanobacteria, Caldilineae, Latescibacteria, Armatimonadetes and Blastocatellia were succseesfully labeled during the degradation of C-PHE, indicating their capacity of utilizing PHE. Co-occurrence network of C-heavy fractions exhibited greater complexity compared with that of C-heavy fractions, revealling an enhancement of bacterial interspecies interactions. Collectivley, this study eluidated the soil microbes involed in the PHE degradation and offered fresh perspectives on the community pattern of potential PHE degrading bacteria.
量化污染土壤中的多环芳烃（PAHs）并鉴定潜在的PAH降解菌对于了解它们的环境归宿和进行生物修复至关重要。然而，在污染土壤环境中负责菲（PHE）分解的微生物群落常常被忽视。在本研究中，通过DNA稳定同位素探测（DNA-SIP），我们发现土壤微生物群可能在PHE降解中起关键作用。在C-PHE和C-PHE微观培养中，PHE去除率分别为98%和99%。随着C-PHE的降解产生了CO。根据16S rRNA基因分析，在用C-PHE处理的“重”DNA组分中存在相对较高比例的未鉴定细菌。在C-PHE降解过程中，肠杆菌目、酸杆菌门、α-变形菌纲、芽孢杆菌科、黄杆菌纲、绿弯菌门、蓝细菌、暖绳菌纲、隐秘杆菌门、装甲菌门和芽单胞菌纲等属被成功标记，表明它们具有利用PHE的能力。与C-重组分的共现网络相比，C-重组分的共现网络表现出更大的复杂性，揭示了细菌种间相互作用的增强。总体而言，本研究阐明了参与PHE降解的土壤微生物，并为潜在PHE降解菌的群落模式提供了新的视角。
