Morgan M S, Meyer P, Holub R, Frank R
Environ Res. 1986 Dec;41(2):546-57. doi: 10.1016/s0013-9351(86)80149-9.
Regional distribution of ventilation and overall function of the lungs were compared in anesthetized, paralyzed, mechanically ventilated dogs exposed to ozone. Three animals were exposed at each concentration (0,0.13,0.22, and 0.45 ppm ozone) for 3 hr. There were no significant changes in pulmonary flow resistance, dynamic compliance, or flow rates derived from maximum forced expiratory maneuvers. The distribution of inspired gas was determined using air containing 13N and a positron camera to follow wash-in in each of 80 or more regions of the lung. The mean wash-in time (T) and its standard deviation did not change in sham-treated dogs, but T increased by 40 to 100% in exposed animals. Dogs showing increases in T also had increased frequency dependence of compliance (correlation significant at P less than 0.05). The changes in 13N wash-in reflected less uniform distribution of ventilation, with the greatest disparity occurring between central and marginal regions. Altered regional mechanical function, in the absence of overall changes, suggested that the initial effect of ozone was on the small airways. The measurement may be a sensitive indicator of acute irritation in the lung periphery.
在暴露于臭氧的麻醉、麻痹且机械通气的犬中，比较了肺部通气的区域分布和整体功能。在每个浓度（0、0.13、0.22和0.45 ppm臭氧）下，对三只动物暴露3小时。肺血流阻力、动态顺应性或最大用力呼气动作得出的流速均无显著变化。使用含13N的空气和正电子相机来确定吸入气体的分布，以追踪肺部80个或更多区域中每个区域的气体吸入情况。假处理犬的平均气体吸入时间（T）及其标准差没有变化，但暴露动物的T增加了40%至100%。T增加的犬的顺应性频率依赖性也增加（相关性在P小于0.05时显著）。13N气体吸入的变化反映了通气分布不太均匀，中央区域和边缘区域之间的差异最大。在没有整体变化的情况下，区域机械功能的改变表明臭氧的初始作用部位是小气道。该测量可能是肺外周急性刺激的敏感指标。
