Wang Qian, Li Xianxu, Yao Xiangfeng, Ding Jia, Zhang Juan, Hu Zhuran, Wang Jinhua, Zhu Lusheng, Wang Jun
College of Resources and Environment, Shandong Agricultural University, Tai'an 271018, PR China.
Shandong Institute for Product Quality Inspection, Jinan 250100, PR China.
Sci Total Environ. 2024 Jan 1;906:167659. doi: 10.1016/j.scitotenv.2023.167659. Epub 2023 Oct 6.
Butyl benzyl phthalate (BBP), a widely used class of plasticizers, has caused considerable concerns due to its widespread detection in various environmental media. However, the potential impact of BBP on the brain and its underlying molecular mechanisms remain poorly understood. In this study, adult zebrafish (Danio rerio) were exposed to 0, 5, 50, and 500 μg/L BBP for 28 days. Elevated levels of both reactive oxygen species and 8-hydroxydeoxyguanosine were observed, indicating the occurrence of oxidative stress and DNA damage. Furthermore, exposure to BBP resulted in neurotoxicity, apoptosis, and histopathological damage within the zebrafish brain. Transcriptome analysis further revealed that Gene Ontology terms associated with muscle contraction were specifically expressed in the brain after BBP exposure. In addition, BBP altered the transcriptome profile of the brain, with 293 genes induced and 511 genes repressed. Kyoto Encyclopedia of Genes and Genomes analysis highlighted the adverse effects of BBP on the complement and coagulation cascades and two cardiomyopathy-related pathways. Taken together, our results revealed that BBP resulted in brain oxidative stress, histological damage, and transcriptome alterations. These findings have the potential to offer novel insights into the adverse outcome pathways of key events in the brain.
邻苯二甲酸丁苄酯（BBP）是一类广泛使用的增塑剂，因其在各种环境介质中被广泛检测到而引起了广泛关注。然而，BBP对大脑的潜在影响及其潜在的分子机制仍知之甚少。在本研究中，成年斑马鱼（Danio rerio）暴露于0、5、50和500μg/L的BBP中28天。观察到活性氧和8-羟基脱氧鸟苷水平升高，表明发生了氧化应激和DNA损伤。此外，暴露于BBP导致斑马鱼大脑内出现神经毒性、细胞凋亡和组织病理学损伤。转录组分析进一步显示，暴露于BBP后，与肌肉收缩相关的基因本体术语在大脑中特异性表达。此外，BBP改变了大脑的转录组谱，诱导了293个基因，抑制了511个基因。京都基因与基因组百科全书分析突出了BBP对补体和凝血级联以及两条与心肌病相关的途径的不利影响。综上所述，我们的结果表明BBP导致大脑氧化应激、组织学损伤和转录组改变。这些发现有可能为大脑关键事件的不良结局途径提供新的见解。
