State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Sci Total Environ. 2024 Jan 10;907:167641. doi: 10.1016/j.scitotenv.2023.167641. Epub 2023 Oct 6.
Artificial sweeteners (AS) are the emerging contaminants with potential toxicity to living organisms. The effects of AS to soil typical invertebrates have not been revealed. In this study, the responses of earthworms (Eisenia fetida) and gut microbial communities to acesulfame-contaminated soils (0.1, 1 and 10 mg kg) were studied using transcriptomics, metabolomics and metagenomics analyses. The fresh weight of earthworms was significantly stimulated by acesulfame at concentrations of 1 mg kg. Sphingolipid metabolism, purine metabolism, cutin, suberine and wax biosynthesis pathways were significantly affected. At 10 mg kg treatment, the amount and weight of cocoons were significantly increased and decreased, respectively, accompanied by the significant disorder of ECM-receptor interaction, and carbon fixation in photosynthetic organisms pathways. Lysosome pathway was significantly affected in all the treatments. Moreover, the acesulfame significantly increased the relative abundance of Bacteroidetes and Mucoromycota, and decreased Proteobacteria in the gut of earthworms. Our multi-level investigation indicated that AS at a relatively low concentration induced toxicity to earthworms and AS pollution has significant environmental risks for soil fauna.
人工甜味剂 (AS) 是具有潜在生物毒性的新兴污染物。AS 对土壤中典型无脊椎动物的影响尚未被揭示。在这项研究中，使用转录组学、代谢组学和宏基因组学分析研究了乙酰磺胺酸污染土壤（0.1、1 和 10 mg/kg）对蚯蚓（赤子爱胜蚓）和肠道微生物群落的影响。在 1 mg/kg 的浓度下，乙酰磺胺酸显著刺激了蚯蚓的鲜重。鞘脂代谢、嘌呤代谢、角质、栓皮和蜡生物合成途径受到显著影响。在 10 mg/kg 处理下，茧的数量和重量分别显著增加和减少，伴随着细胞外基质-受体相互作用和光合生物碳固定途径的显著紊乱。溶酶体途径在所有处理中均受到显著影响。此外，乙酰磺胺酸显著增加了蚯蚓肠道中拟杆菌门和毛霉门的相对丰度，减少了变形菌门的相对丰度。我们的多层次调查表明，AS 在相对较低的浓度下诱导了对蚯蚓的毒性，AS 污染对土壤无脊椎动物具有显著的环境风险。
