Toyohara T, Nada O, Ikeda K
Eur Surg Res. 1986;18(6):349-55. doi: 10.1159/000128546.
Sympathetic innervation was examined by fluorescence histochemistry in the aganglionic colon from 13 patients with Hirschsprung's disease. The 2 aganglionic colons subjected to ischemia were virtually devoid of fluorescent noradrenergic nerves. However, the sympathetic nerves of the ischemic colons fluoresced brightly after an in vitro loading of noradrenaline. Such being the case, we investigated the influence of ischemia on the sympathetic nerves in the distal colon of rats. Even 1 h of ischemia depleted the fluorescence of sympathetic nerves in the rat distal colon. Loading of noradrenaline resulted in restoration of the fluorescence in the sympathetic nerves in the distal colon subjected to 2, but not 3 h, of ischemia. The dense noradrenergic innervation, together with the occurrence of small nerve bundles, was characteristically evident in the longitudinal muscle of the distal aganglionic colon from children with Hirschsprung's disease.
通过荧光组织化学方法对13例先天性巨结肠患者的无神经节结肠的交感神经支配进行了研究。2例经历缺血的无神经节结肠几乎没有荧光去甲肾上腺素能神经。然而，在体外加载去甲肾上腺素后，缺血结肠的交感神经发出明亮的荧光。鉴于此，我们研究了缺血对大鼠远端结肠交感神经的影响。即使缺血1小时也会使大鼠远端结肠交感神经的荧光消失。加载去甲肾上腺素可使经历2小时而非3小时缺血的远端结肠交感神经的荧光恢复。在先天性巨结肠患儿的远端无神经节结肠的纵行肌中，密集的去甲肾上腺素能神经支配以及小神经束的出现具有典型的明显特征。
