口腔扁平苔藓中T细胞亚群（CD4和CD8）的免疫组织化学表达

Immunohistochemical expression of T-cell subsets (CD4 and CD8) in oral lichen planus.

作者信息

Rassol Hiba Jassim, Zaidan Taghreed Fadhil

机构信息

Department of Oral Diagnosis, College of Dentistry, University of Baghdad, Baghdad, Iraq.

Department of Dentistry, Al-Turath University College, Baghdad, Iraq.

出版信息

Pan Afr Med J. 2023 Aug 2;45:147. doi: 10.11604/pamj.2023.45.147.38629. eCollection 2023.

DOI:10.11604/pamj.2023.45.147.38629

PMID:37808434

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10559156/

Abstract

INTRODUCTION

oral lichen planus (OLP) is a common oral mucosal disease with various clinical manifestations. The most predominant types are reticular and erosive. Despite extensive research on the causes of OLP, the exact etiology remains unclear. However, it is believed that a T-cell-mediated response, which triggers the apoptosis of oral epithelial cells, may contribute to the development of this disorder. This study aims to investigate the different types of T-cells (specifically CD4 and CD8) present in OLP tissue samples. By using immunohistochemistry, the expressions of cluster of differentiation 4 (CD4) and cluster of differentiation 8 (CD8) will be evaluated in biopsy samples taken from OLP patients who exhibit various clinical presentations.

METHODS

this study was a retrospective analysis study. Oral lichen planus was established histologically in forty paraffin-embedded tissue samples. Blocks of OLP were diagnosed and characterized as reticular or erosive. Immunohistochemical staining was conducted using a monoclonal antibody for (CD4) and a polyclonal antibody for CD8. Semi-quantitative techniques were used to analyze the patterns of positively stained cells. forty biopsies of OLP cases were obtained from 24 females and 16 males. The mean age was (49.15±11.39) years. Using an immunohistochemical method, the proportion of CD4 expression: CD8 expression among the epithelial-connective tissue interface was shown to be 24 (60%) cases with a predominance of CD8, 9 (22.5%) cases with no difference, and only 7 (17.5%) cases with a predominance of CD4. The proportion of CD4: CD8 among perivascular parts was shown to be 8 (20%) cases with a predominance of CD8, 20 (50%) cases with no difference, while only 12 (30%) cases had a predominance of CD4. The CD4 perivascular expression was significantly stronger in (71.4%) of erosive OLP than in reticular cases.

CONCLUSION

T-cell subsets (CD4 and CD8) were found in the OLP infiltrates. The correlation may have contributed to the pathogenesis of OLP.

摘要

引言

口腔扁平苔藓（OLP）是一种常见的口腔黏膜疾病，有多种临床表现。最主要的类型是网状和糜烂型。尽管对OLP的病因进行了广泛研究，但其确切病因仍不清楚。然而，据信由T细胞介导的反应触发口腔上皮细胞凋亡，可能促使这种疾病的发展。本研究旨在调查OLP组织样本中存在的不同类型T细胞（特别是CD4和CD8）。通过免疫组织化学方法，将在取自表现出各种临床表现的OLP患者的活检样本中评估分化簇4（CD4）和分化簇8（CD8）的表达。

方法

本研究为回顾性分析研究。在40个石蜡包埋组织样本中通过组织学方法确诊为口腔扁平苔藓。OLP组织块被诊断并分为网状或糜烂型。使用针对CD4的单克隆抗体和针对CD8的多克隆抗体进行免疫组织化学染色。采用半定量技术分析阳性染色细胞的模式。40例OLP活检样本取自24名女性和16名男性。平均年龄为（49.15±11.39）岁。采用免疫组织化学方法显示，上皮-结缔组织界面处CD4表达与CD8表达的比例为：24例（60%）以CD8为主，9例（22.5%）无差异，仅7例（17.5%）以CD4为主。血管周围部分CD4与CD8的比例为：8例（20%）以CD8为主，20例（50%）无差异，而仅12例（30%）以CD4为主。糜烂型OLP中71.4%的血管周围CD4表达明显强于网状型病例。