Medical Laboratory Center, Jinjiang Municipal Hospital, Jinjiang, Quanzhou, China.
Department of Ophthalmology, Jinjiang Municipal Hospital, Jinjiang, Quanzhou, China.
Front Cell Infect Microbiol. 2023 Sep 21;13:1268668. doi: 10.3389/fcimb.2023.1268668. eCollection 2023.
We report a case of keratitis, first detected in the laboratory, which is from an 81-year-old female patient with a 13-year history of recurrent keratitis after eye injuries. This patient underwent anterior chamber irrigation of the right eye, and autologous conjunctival flap covering plus medication, and the corneal ulcer healed. She recovered well after continuing with the medication for half a year. The patient was not immune-compromised. Complex eye diseases such as blurred vision and cataracts caused by advanced age, delayed symptoms caused by slow growth of and low-grade inflammation, difficulty with laboratory culture, repeated medication use, and repeated illnesses were the main reasons for the prolonged failure to clarify the etiology of this case. Morphological examination provided important clues for the initial discovery of pathogenic bacteria. Remind to pay attention to the morphology of Mycobacterium under Gram staining and Rui's Giemsa staining. Acid fast staining and Deoxyribonucleic Acid(DNA) microarray chip method can be used as indicators for rapid diagnosis of Mycobacterium species.
我们报告了一例角膜炎病例，该病例最初是在实验室中发现的，患者为 81 岁女性，13 年前眼部受伤后反复发作角膜炎。该患者接受了右眼前房冲洗，自体结膜瓣覆盖加药物治疗，角膜溃疡愈合。继续用药半年后，她恢复良好。患者无免疫功能低下。由于年龄较大导致的复杂眼部疾病如视力模糊和白内障、生长缓慢和低度炎症导致的症状延迟、实验室培养困难、反复用药和反复发病是导致该病例病因长期未能明确的主要原因。形态学检查为最初发现病原菌提供了重要线索。提醒注意革兰氏染色和瑞氏吉姆萨染色下分枝杆菌的形态。抗酸染色和脱氧核糖核酸（DNA）微阵列芯片法可作为快速诊断分枝杆菌属的指标。
