Munske G R, Magnuson J A, Barbet A F
Exp Parasitol. 1986 Dec;62(3):423-9. doi: 10.1016/0014-4894(86)90051-2.
Fluidity of the plasma membrane of Trypanosoma brucei brucei has been examined with fluorescence and electron spin resonance spectroscopy. Fluorescent probes 1,6-diphenyl-1,3,5-hexatriene and 8-anilino-1-naphthalene sulfonate and the spin label probe 5-doxyl stearate have been employed to examine fluidity under a variety of conditions. The temperature dependence of 8-anilino-1-naphthalene sulfonate polarization and of the order parameter S for 5-doxyl stearate reveals phase alterations near 30 C. 1,6-Diphenyl-1,3,5-hexatriene polarization shows that proteolysis of the surface glycoprotein with trypsin increases fluidity but treatment with human serum which is trypanocidal produces no detectable change in membrane fluidity.
已通过荧光和电子自旋共振光谱法检测了布氏布氏锥虫质膜的流动性。使用荧光探针1,6-二苯基-1,3,5-己三烯和8-苯胺基-1-萘磺酸盐以及自旋标记探针5-硬脂酰氧基硬脂酸盐在各种条件下检测流动性。8-苯胺基-1-萘磺酸盐极化和5-硬脂酰氧基硬脂酸盐的序参数S对温度的依赖性揭示了在30℃附近的相变。1,6-二苯基-1,3,5-己三烯极化表明用胰蛋白酶对表面糖蛋白进行蛋白水解会增加流动性，但用具有杀锥虫作用的人血清处理不会使膜流动性产生可检测到的变化。
