Takano E, Maki M, Hatanaka M, Mori H, Zenita K, Sakihama T, Kannagi R, Marti T, Titani K, Murachi T
FEBS Lett. 1986 Nov 24;208(2):199-202. doi: 10.1016/0014-5793(86)81017-1.
A clone of complementary DNA (cDNA) coding for pig heart calpastatin was isolated using synthetic oligonucleotide probes. The amino acid sequence deduced from the nucleotide sequence revealed the occurrence of a repetitive sequence at the interval of 140 amino acids, substantiating the multidomain structure of calpastatin. A portion of the sequence of 251 amino acid residues predicted for pig heart calpastatin (107 kDa) was found to be identical with that of a peptide fragment derived from pig erythrocyte calpastatin (68 kDa) and sequenced by Edman degradation.
利用合成寡核苷酸探针分离出编码猪心钙蛋白酶抑制蛋白的互补DNA（cDNA）克隆。从核苷酸序列推导的氨基酸序列显示，在每140个氨基酸的间隔处存在重复序列，证实了钙蛋白酶抑制蛋白的多结构域结构。猪心钙蛋白酶抑制蛋白（107 kDa）预测的251个氨基酸残基的部分序列，被发现与源自猪红细胞钙蛋白酶抑制蛋白（68 kDa）并通过埃德曼降解测序的肽片段序列相同。
