通过互补DNA克隆证明猪钙蛋白酶抑制蛋白重复结构域结构的证据。

Evidence for the repetitive domain structure of pig calpastatin as demonstrated by cloning of complementary DNA.

作者信息

Takano E, Maki M, Hatanaka M, Mori H, Zenita K, Sakihama T, Kannagi R, Marti T, Titani K, Murachi T

出版信息

FEBS Lett. 1986 Nov 24;208(2):199-202. doi: 10.1016/0014-5793(86)81017-1.

Abstract

A clone of complementary DNA (cDNA) coding for pig heart calpastatin was isolated using synthetic oligonucleotide probes. The amino acid sequence deduced from the nucleotide sequence revealed the occurrence of a repetitive sequence at the interval of 140 amino acids, substantiating the multidomain structure of calpastatin. A portion of the sequence of 251 amino acid residues predicted for pig heart calpastatin (107 kDa) was found to be identical with that of a peptide fragment derived from pig erythrocyte calpastatin (68 kDa) and sequenced by Edman degradation.

摘要

利用合成寡核苷酸探针分离出编码猪心钙蛋白酶抑制蛋白的互补DNA（cDNA）克隆。从核苷酸序列推导的氨基酸序列显示，在每140个氨基酸的间隔处存在重复序列，证实了钙蛋白酶抑制蛋白的多结构域结构。猪心钙蛋白酶抑制蛋白（107 kDa）预测的251个氨基酸残基的部分序列，被发现与源自猪红细胞钙蛋白酶抑制蛋白（68 kDa）并通过埃德曼降解测序的肽片段序列相同。